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      Characterization of rotavirus guanylyltransferase activity associated with polypeptide VP3.

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          Abstract

          Rotaviruses transcribe mRNA containing a 7mGpppGmp cap at the 5' end in vitro. Guanylyltransferase activity associated with the viral particle was detected by SDS-PAGE due to the formation of a nucleotide-enzyme complex when the virus was incubated with [alpha-32P]GTP. Using purified viral particles it was shown that only the core polypeptide VP3 exhibits the ability to form a complex with the nucleotide. The reaction is specific for GTP or dGTP when Mg2+ is used as a cofactor. The reaction also depends on the incubation temperature and the pH, as described for other guanylyltransferases. The GMP-VP3 complex transfers the GMP to pyrophosphate, synthesizing GTP or GDP, resulting in the formation of a GpppG cap. These properties of the complex allowed the core polypeptide VP3 to be identified as the rotavirus guanylyltransferase.

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          Author and article information

          Journal
          J. Gen. Virol.
          The Journal of general virology
          Microbiology Society
          0022-1317
          0022-1317
          Feb 1991
          : 72 ( Pt 2)
          Affiliations
          [1 ] Unit of Virology, Instituto de Nutrición y Tecnología de los Alimentos, Universidad de Chile, Santiago.
          Article
          10.1099/0022-1317-72-2-325
          1704411

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