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      Hematological parameters in Nile Tilápia, Oreochromis niloticus exposed to sub-letal concentrations of mercury

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          Abstract

          Mercury toxicity in tilapia, Oreochromis niloticus, (Linnaeus, 1758) was investigated by the hematological parameters after long-term (14 days) exposure to various Hg concentrations (0.02, 0.002, 0.0002mg/L Hg). Test groups were set up with three replicates for each concentration, plus the control group. Blood samples were collected from six individuals for each concentration at 0, 3, 7, 10 and 14 days of exposure. The hematological parameters analyzed were: total red blood cell count (RBC), hemoglobin concentration (Hb), hematocrit (Ht), mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH), mean corpuscular hemoglobin concentration (MCHC), total white blood cell count (WBC) and differential leukocyte counts and total thrombocyte count (Tr). There were no significant differences among the mean hematological values at the different Hg concentrations indicating that Hg at the concentrations studied was not toxic to tilapia.

          Translated abstract

          A toxicidade do mercúrio foi avaliada em tilápia, Oreochromis niloticus (Linnaues, 1758) através da análise dos parâmetros hematológicos após exposição a diferentes concentrações sub-letais, durante um período de 14 dias. O bioensaio foi conduzido no laboratório de toxicologia do Instituto de Pesca, SP. Foram utilizados alevinos (12.44 ± 0.84 cm, e 27.13 ± 4.67 g) e aquários com capacidade para 50 litros e preenchidos com água declorada e mais a quantidade de solução de mercúrio (HgCl2) correspondendo as seguintes concentrações: 0,02; 0,002; 0.0002 mg.L-1 Hg. Foram utilizadas 3 repetições de cada concentração e grupo controle. Amostras de sangue foram coletadas de seis animais de cada concentração nos tempos 0, 3, 7, 10 e 14 dias de exposição. Foram avaliados: a contagem de eritrócitos (RBC), concentração de hemoglobina (Hb), hematócrito (Ht), volume corpuscular médio (VCM), hemoglobina corpuscular média (HCM) e concentração de hemoglobina corpuscular média (CHCM), trombócitos totais (Tr), contagem diferencial e total de leucócitos (Lc). Os resultados demonstram que as concentrações de Hg testadas, não alteraram significativamente os parâmetros hematológicos, permitindo concluir que a quantidade de Hg na água não foram suficientes para afetar o quadro hematológico de Oreochromis niloticus.

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          Most cited references40

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          A New Blood Diluent for Counting the Erythrocytes and Leucocytes of the Chicken

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            Fish Diseases: Diagnosis and Treatment

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              Acid and alkaline phosphatase activities and pathological changes induced in Tilapia fish (Oreochromis sp.) exposed subchronically to microcystins from toxic cyanobacterial blooms under laboratory conditions.

              The effects of microcystins (MCs) from cyanobacterial cells on the enzymatic activities of acid and alkaline phosphatases (ACP and ALP) from liver, kidney and gill tissues, and the histopathological changes in freshwater Tilapia fish (Oreochromis sp.) were investigated under laboratory conditions. Fish were exposed to cyanobacterial cells (60.0 microg MC-LR/fish per day) through their diet at different exposure times (14 and 21 days). The cells were fed to the fish in two types of oral administration: mixed with a commercial fish food or crushed into a commercial fish food so that the toxins were released. ACP and ALP activities changed in response to MCs in a time-dependent manner, and these changes were more prominent in liver and kidney. The way the MCs were administered had no influence on the biochemical parameters. Similarly, the most severe histopathological changes were observed in the same two organs, although the gills and intestines were also affected. The parenchymal architecture of the liver was dissolved, and round hepatocytes with the appearance of pyknotic nuclei were detected. Kidney lesions consisted of the dilation of Bowman's space and necrotic epithelial cells with pyknotic nuclei in the tubules. These findings suggest that low and repeated doses of MC-LR from cyanobacterial cells induce toxicity in tilapia fish although no adverse effects were detected.

                Author and article information

                Journal
                babt
                Brazilian Archives of Biology and Technology
                Braz. arch. biol. technol.
                Instituto de Tecnologia do Paraná - Tecpar (Curitiba, PR, Brazil )
                1516-8913
                1678-4324
                July 2007
                : 50
                : 4
                : 619-626
                Affiliations
                [01] orgnameUNESP orgdiv1Centro de Aqüicultura niltonmi@ 123456caunesp.unesp.br
                [02] São Paulo SP orgnameInstituto de Pesca SAA-APTA orgdiv1Instituto de Pesca SAA-APTA Brasil
                Article
                S1516-89132007000400007 S1516-8913(07)05000407
                10.1590/S1516-89132007000400007
                157d996d-e576-45a2-863c-987804d6ff49

                This work is licensed under a Creative Commons Attribution 4.0 International License.

                History
                : 15 August 2005
                : 14 March 2007
                : 01 June 2006
                Page count
                Figures: 0, Tables: 0, Equations: 0, References: 40, Pages: 8
                Product

                SciELO Brazil

                Categories
                Human and Animal Health

                hematology,fish,Oreochromis niloticus,Mercury,heavy metal pollution

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