Abortive infection of Escherichia coli F+ cells by bacteriophage T7 requires ribosomal misreading

Abstract

The use of different precisely mapped T3/T7 recombinants strengthens the conclusion that abortive infection by T7 of F plasmid-carrying cells is due to the nucleotide sequence at the end of the T7 gene 1. Furthermore, we demonstrate that the exclusion requires suppression of ochre stop codons, a phenomenon that occurs with low frequency in wild-type cells due to ribosomal misreading. The introduction of rspL mutations in which ribosomal misreading is reduced alleviates the exclusion and the presence of ochre tRNA suppressors increases its severity.

Journal

Title: Journal of Molecular Biology

Abbreviated Title: Journal of Molecular Biology

Publisher: Elsevier BV

ISSN (Print): 00222836

Publication date Created: March 1987

Publication date (Print): March 1987

Volume: 194

Issue: 2

Pages: 349-352

Article

DOI: 10.1016/0022-2836(87)90382-2

PubMed ID: 3302277

Copyright statement: © 1987

License:

https://www.elsevier.com/tdm/userlicense/1.0/

Product

Self URI (article page): https://linkinghub.elsevier.com/retrieve/pii/0022283687903822