There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.
The use of different precisely mapped T3/T7 recombinants strengthens the conclusion
that abortive infection by T7 of F plasmid-carrying cells is due to the nucleotide
sequence at the end of the T7 gene 1. Furthermore, we demonstrate that the exclusion
requires suppression of ochre stop codons, a phenomenon that occurs with low frequency
in wild-type cells due to ribosomal misreading. The introduction of rspL mutations
in which ribosomal misreading is reduced alleviates the exclusion and the presence
of ochre tRNA suppressors increases its severity.