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      Abortive infection of Escherichia coli F+ cells by bacteriophage T7 requires ribosomal misreading

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      Journal of Molecular Biology

      Elsevier BV

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          Abstract

          The use of different precisely mapped T3/T7 recombinants strengthens the conclusion that abortive infection by T7 of F plasmid-carrying cells is due to the nucleotide sequence at the end of the T7 gene 1. Furthermore, we demonstrate that the exclusion requires suppression of ochre stop codons, a phenomenon that occurs with low frequency in wild-type cells due to ribosomal misreading. The introduction of rspL mutations in which ribosomal misreading is reduced alleviates the exclusion and the presence of ochre tRNA suppressors increases its severity.

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          Author and article information

          Journal
          Journal of Molecular Biology
          Journal of Molecular Biology
          Elsevier BV
          00222836
          March 1987
          March 1987
          : 194
          : 2
          : 349-352
          Article
          10.1016/0022-2836(87)90382-2
          3302277
          © 1987

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