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Abstract
This study is designed to explore the mechanism by which long non-coding RNA (lncRNA)
antisense non-coding RNA in the INK4 locus (ANRIL) plays a pathogenic role in uric
acid nephropathy (UAN). The expressions of ANRIL, miR-122-5p, BRCA1-BRCA2-containing
complex subunit 3 (BRCC3) and NOD-like receptor protein 3 (NLRP3) were determined
in UAN patients and uric acid-treated HK-2 cells by qRT-PCR. Protein levels of BRCC3
and NLRP3 were examined by western blot. The levels of inflammatory cytokines were
quantified by ELISA. CCK-8 assay was used to assess cell viability. Apoptosis was
detected by Annexin V-FITC/PI double-labeled flow cytometry and TUNEL assay. The interaction
between ANRIL, miR-122-5p and BRCC3 were studied using luciferase reporter assay.
The role of ANRIL in renal injury was evaluated in experimental rats. ANRIL and BRCC3
were highly expressed while miR-122-5p was down-regulated in serum of UAN patients
and uric acid-treated tubular epithelial cells. Luciferase reporter assay and in vitro
rescue experiment confirmed that ANRIL promoted NLRP3 inflammasome activation by up-regulating
BRCC3 expression via sponging miR-122-5p. Furthermore, in vivo experiment validated
that knockdown of ANRIL alleviated renal injury of UAN rats. ANRIL exerted pathogenic
effect in UAN to promote NLRP3 inflammasome activation via miR-122-5p/BRCC3 axis.