Spotted Sea Bass ( Lateolabrax maculatus) cftr, nkcc1a, nkcc1b and nkcc2: Genome-Wide Identification, Characterization and Expression Analysis Under Salinity Stress

Abstract

The Na ⁺/K ⁺/2Cl ⁻ cotransporter (NKCC) and the cystic fibrosis transmembrane conductance regulator (CFTR) proteins play crucial roles in the transportation of Na ⁺ and Cl ⁻. In this study, we identified cftr, nkcc1a, nkcc1b and nkcc2 in spotted sea bass ( Lateolabrax maculatus) genomic and transcriptomic databases. We also characterized these genes via phylogenetic and structural analyses. The results showed that both cftr and nkcc were highly conservative in L. maculatus. Quantitative real-time polymerase chain reaction (qRT-PCR) analysis in ten tissues showed that cftr, nkcc1a and nkcc2 highly express in osmoregulatory organs such as gill, kidney and intestine. Furthermore, the expressions of cftr and nkcc1a in gill as well as nkcc2 in intestine were up-regulated by high salinity, indicating that these genes function potentially in osmoregulation. Our findings provided the insights into the cftr and nkcc functions in euryhaline teleost.

Journal

Journal ID (publisher-id): JOUC

Title: Journal of Ocean University of China

Publisher: Science Press and Springer (China )

ISSN (Electronic): 1672-5182

Publication date (Electronic): 12 November 2019

Publication date (Print): 01 December 2019

Volume: 18

Issue: 6

Pages: 1470-1480

Affiliations

¹College of Fisheries, Ocean University of China, Qingdao 266003, China

²The Key Laboratory of Mariculture of Ministry of Education, Ocean University of China, Qingdao 266003, China

Author notes

*Corresponding author: LI Yun

Article

Publisher ID: s11802-019-4114-0

DOI: 10.1007/s11802-019-4114-0

Copyright statement: Copyright © Ocean University of China, Science Press and Springer-Verlag GmbH Germany 2019.

License:

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Product

Self URI (journal-page): https://www.springer.com/journal/11802