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      Método no invasivo de diagnóstico de gestación en venadas Odocoileus virginianus Translated title: Non-Invasive Method for Pregnancy Diagnosis in Odocoileus Virginianus

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          Abstract

          Objetivo: Valorar la eficiencia del diagnóstico de gestación mediante la estimación de niveles de progestinas fecales en venadas cola blanca O. virginianus en la época no reproductiva. Materiales y métodos: Se analizaron muestras sanguíneas y heces de nueve venadas cola blanca (VCB), semanalmente, durante dos meses en la época reproductiva. Adicionalmente, las VCB fueron expuestas a macho durante un mes duranteépoca reproductiva, y se colectaron heces dos veces fuera de esta, para medir los niveles de metabolitos de progestinas. Se determinó el valor de corte para diagnosticar gestación a partir de los niveles de metabolitos de progestinas fecales en fase luteal y folicular. La progesterona sanguínea fue medida por radioinmunoanálisis, y se correlacionó con los niveles de progestinas fecales. Los diagnósticos de gestación fueron relacionados con los partos. Resultados: La correlación total entre niveles de progestinas sanguíneas y metabolitos de progestinas fecales fue 0,37 (p<0,01). La variación de la correlación individual fue 0,104 hasta 0,99. El valor de corte para diagnosticar gestación fue 4000 ng/g en heces. La eficiencia del diagnóstico de gestación fue 77,78 %; el valor predictivo del diagnóstico positivo fue 75 %, y el negativo fue 80 %, respectivamente. Conclusiones: Es posible diagnosticar gestación fuera de la época de reproducción mediante la estimación de los niveles de metabolitos de progestinas fecales, en VCB en cautiverio, tomando como valor de corte 4000 ng/g de heces.

          Translated abstract

          Objective: To value the efficiency of pregnancy diagnosis by estimating levels of fecal progestins in white-tailed deer O. virginianus outside the reproductive season. Materials and methods: Blood and feces samples of nine White tailed-deer (WTD) were analyzed weekly for two months in reproductive season. Additionally WTD were exposed to a male during a month in reproductive season and feces were collected twice in non-reproductive season, in order to estimate progestins metabolites levels. The cut value for pregnancy diagnosis from levels of fecal progestins metabolites in luteal and follicular phases of WTD, was determined. Blood progesterone levels were measured by radioimmuno analysis and were correlated with the fecal progestins levels. Pregnancy diagnosis was related with the deliveries. Results: Total correlation coefficient between blood progestins levels and fecal metabolites progestins was 0.37 (p<0.01). Individual correlation coefficient variation was 0.104 to 0.99. Cut off value for pregnancy diagnosis was 4000 ng/g in feces. Efficiency for pregnancy diagnosis was 77.78%, predictive positive value was 75% and the negative was 80% respectively. Conclusions: It is possible to carry out pregnancy diagnosis in non-reproductive season, through quantification of fecal progestin metabolites levels, in WTD in captivity, taking as cut value 4000 ng/g feces.

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          Most cited references34

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          Monitoring Reproduction in Captive Moose Using Urinary and Fecal Steroid Metabolites

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            Serum and fecal steroid analysis of ovulation, pregnancy, and parturition in the black rhinoceros (Diceros bicornis)

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              Fecal progestins during pregnancy and postpartum periods of captive red brocket deer (Mazama americana).

              This study aimed to validate the enzyme immunoassay (EIA) for fecal progestin quantification of the species Mazama americana, define its excretion profile during periods of gestation and postpartum and determine the gestation period and resumption of postpartum ovarian activity in this species in captivity. Fecal samples were collected twice a week during gestation and every day in the postpartum period, and analyzed using EIA. The mean concentrations (±SEM) of fecal progestins during gestation were 2180.0±299.1ng/g in early pregnancy (week 1-11), 3271.4±406.9ng/g in middle pregnancy (week 12-22) and 5592.0±1125.8ng/g in late pregnancy (week 23-32). The gestation period determined for the species was 220.9±1.2 days. The concentration of progestins reached its peak prior to parturition and returned to baseline levels in 4±0.31 days after parturition. In the postpartum period, the mean concentrations of fecal progestins were 1564.2±182.6ng/g in the interval between parturition and resumption of ovarian activity, 469.8±24.5ng/g in the inter-luteal phase and 2401.7±318.5ng/g during the luteal phase, such that the postpartum period and the luteal phase differed from the inter-luteal phase. Fecal progestin profiling permitted the detection of ovulation 26.9±3.4 days after parturition in all the hinds studied and estimation of the mean duration of the estrous cycle, 21.3±1.1 days. Analysis established that concentrations of progestins above 3038.76ng/g diagnosed pregnancy, a value determined from the week 12 of gestation. Moreover, the quantification of fecal progestins by EIA proved to be an important tool for noninvasive endocrine monitoring and to obtain reproductive data on the species M. americana in captivity. Copyright © 2013 Elsevier B.V. All rights reserved.
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                Author and article information

                Contributors
                Role: ND
                Role: ND
                Role: ND
                Role: ND
                Role: ND
                Journal
                cide
                Ciencia en Desarrollo
                Ciencia en Desarrollo
                Universidad Pedagógica y Tecnológica de Colombia (Tunja )
                0121-7488
                January 2015
                : 6
                : 1
                : 9-16
                Affiliations
                [1 ] Universidad Autónoma de Yucatán México
                [2 ] Parque Zoológico del Centenario México
                [3 ] Universidad Pedagógica y Tecnológica de Colombia Boyacá
                Article
                S0121-74882015000100002
                161832da-2d05-4f53-bac8-29cdcacd0050

                http://creativecommons.org/licenses/by/4.0/

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                SciELO Colombia

                Self URI (journal page): http://www.scielo.org.co/scielo.php?script=sci_serial&pid=0121-7488&lng=en
                Categories
                SOCIAL SCIENCES, INTERDISCIPLINARY

                Sociology
                Steroid Metabolites,Feces,Deer,Reproduction,Pregnancy Diagnosis,Metabolitos esteroides,Heces,Cérvidos,Reproducción de venadas,Diagnóstico de gestación

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