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      Diffraction tomography with Fourier ptychography

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          Abstract

          This paper presents a technique to image the complex index of refraction of a sample across three dimensions. The only required hardware is a standard microscope and an array of LEDs. The method, termed Fourier ptychographic tomography (FPT), first captures a sequence of intensity-only images of a sample under angularly varying illumination. Then, using principles from ptychography and diffraction tomography, it computationally solves for the sample structure in three dimensions. The experimental microscope demonstrates a lateral spatial resolution of 0.39 μm and an axial resolution of 3.7 μm at the Nyquist–Shannon sampling limit (0.54 and 5.0 μm at the Sparrow limit, respectively) across a total imaging depth of 110 μm. Unlike competing methods, this technique quantitatively measures the volumetric refractive index of primarily transparent and contiguous sample features without the need for interferometry or any moving parts. Wide field-of-view reconstructions of thick biological specimens suggest potential applications in pathology and developmental biology.

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          Author and article information

          Journal
          101643595
          43136
          Optica
          Optica
          Optica
          2334-2536
          10 December 2016
          27 July 2016
          August 2016
          21 July 2017
          : 3
          : 8
          : 827-835
          Affiliations
          [1 ]Department of Electrical Engineering, California Institute of Technology, Pasadena, California 91125, USA
          [2 ]Bioimaging and Neurophotonics Lab, NeuroCure Cluster of Excellence, Charité Berlin, Humboldt University, Berlin 10117, Germany
          [3 ]Department of Biomedical Engineering, University of Connecticut, Storrs, Connecticut 06269, USA
          Author notes
          [* ]Corresponding author: roarke@ 123456caltech.edu
          Article
          PMC5521281 PMC5521281 5521281 nihpa835228
          10.1364/OPTICA.3.000827
          5521281
          28736737
          1646c581-6590-4d2e-9a12-716d2aed936c
          History
          Categories
          Article

          (110.6955) Tomographic imaging,(180.6900) Three-dimensional microscopy

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