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      DNA binding preferences of PPAR alpha/RXR alpha heterodimers.

      1 , ,
      Biochemical and biophysical research communications
      Elsevier BV

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          Abstract

          The regulatory elements mediating the transcriptional effects of the Peroxisome Proliferator Activated Receptor (PPAR)/Retinoid X Receptor heterodimers consist of a direct repeat of a variant of the consensus hexamer AGGTCA with an interspacing of 1 basepair (DR1). A binding site selection was performed to investigate whether any further constraints for PPAR/RXR binding to DR1 elements exist and/or whether other high affinity binding sites for these heterodimers can be identified. One half of the recovered sequences contained two hexamers related to the consensus halfsite organised as DR1, DR2, PAL0 or as DR3, in diminishing order of frequency. The other binding sites consisted of three hexamer repeats with the number of interspacing bases varying between 0 and 7. An element with three consecutive hexamer sequences each spaced by 1 basepair was most efficient in mediating the effects of peroxisome proliferators. The results indicate that the upstream flanking sequence of a DR1 differentially influences the binding of PPAR alpha/RXR alpha heterodimers and of RXR alpha homodimers.

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          Author and article information

          Journal
          Biochem. Biophys. Res. Commun.
          Biochemical and biophysical research communications
          Elsevier BV
          0006-291X
          0006-291X
          Apr 07 1997
          : 233
          : 1
          Affiliations
          [1 ] Laboratory for Clinical Chemistry, Faculty of Pharmaceutical Sciences, Catholic University of Leuven, Belgium.
          Article
          S0006-291X(97)96395-6
          10.1006/bbrc.1997.6395
          9144402
          16cfe2f4-0791-468c-9129-5189c10c6a63
          History

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