6
views
0
recommends
+1 Recommend
0 collections
    0
    shares
      • Record: found
      • Abstract: found
      • Article: not found

      An endoxylanase rapidly hydrolyzes xylan into major product xylobiose via transglycosylation of xylose to xylotriose or xylotetraose.

      Read this article at

      ScienceOpenPublisherPubMed
      Bookmark
          There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.

          Abstract

          Here, we proposed an effective strategy to enhance a novel endoxylanase (Taxy11) activity and elucidated an efficient catalysis mechanism to produce xylooligosaccharides (XOSs). Codon optimization and recruitment of natural propeptide in Pichia pastoris resulted in achievement of Taxy11 activity to 1405.65 ± 51.24 U/mL. Analysis of action mode reveals that Taxy11 requires at least three xylose (xylotriose) residues for hydrolysis to yield xylobiose. Results of site-directed mutagenesis indicate that residues Glu119, Glu210, and Asp53 of Taxy11 are key catalytic sites, while Asp203 plays an auxiliary role. The novel mechanism whereby Taxy11 catalyzes conversion of xylan or XOSs into major product xylobiose involves transglycosylation of xylose to xylotriose or xylotetraose as substrate, to form xylotetraose or xylopentaose intermediate, respectively. Taxy11 displayed highly hydrolytic activity toward corncob xylan, producing 50.44 % of xylobiose within 0.5 h. This work provides a cost-effective and sustainable way to produce value-added biomolecules XOSs (xylobiose-enriched) from agricultural waste.

          Related collections

          Author and article information

          Journal
          Carbohydr Polym
          Carbohydrate polymers
          Elsevier BV
          1879-1344
          0144-8617
          Jun 01 2020
          : 237
          Affiliations
          [1 ] Beijing Advanced Innovation Center for Food Nutrition and Human Health, State Key Laboratory of Agro-Biotechnology, College of Biological Sciences, China Agricultural University, Beijing, 100193, China. Electronic address: 18811068358@163.com.
          [2 ] Beijing Advanced Innovation Center for Food Nutrition and Human Health, State Key Laboratory of Agro-Biotechnology, College of Biological Sciences, China Agricultural University, Beijing, 100193, China. Electronic address: nnsong77@163.com.
          [3 ] Beijing Advanced Innovation Center for Food Nutrition and Human Health, State Key Laboratory of Agro-Biotechnology, College of Biological Sciences, China Agricultural University, Beijing, 100193, China. Electronic address: abdul_9090@yahoo.com.
          [4 ] Beijing Advanced Innovation Center for Food Nutrition and Human Health, State Key Laboratory of Agro-Biotechnology, College of Biological Sciences, China Agricultural University, Beijing, 100193, China. Electronic address: ljq0802040421@163.com.
          [5 ] Beijing Advanced Innovation Center for Food Nutrition and Human Health, State Key Laboratory of Agro-Biotechnology, College of Biological Sciences, China Agricultural University, Beijing, 100193, China. Electronic address: 15501017617@163.com.
          [6 ] Beijing Advanced Innovation Center for Food Nutrition and Human Health, State Key Laboratory of Agro-Biotechnology, College of Biological Sciences, China Agricultural University, Beijing, 100193, China. Electronic address: 15801029792@163.com.
          [7 ] State Key Laboratory of Animal Nutrition, China Agricultural University, Beijing, China. Electronic address: caoyh@cau.edu.cn.
          [8 ] Beijing Advanced Innovation Center for Food Nutrition and Human Health, State Key Laboratory of Agro-Biotechnology, College of Biological Sciences, China Agricultural University, Beijing, 100193, China. Electronic address: jiangwei01@cau.edu.cn.
          Article
          S0144-8617(20)30295-2
          10.1016/j.carbpol.2020.116121
          32241400
          16e6ed35-7a3f-48ee-b5d9-986dea28a0ab
          Copyright © 2020 Elsevier Ltd. All rights reserved.
          History

          Action mode,Biomass conversion,Catalysis mechanism,Improved expression,Sodium carboxymethyl cellulose (PubChem CID: 23706213),Xylanolytic enzyme,Xylobiose,Xylobiose (PubChem CID: 126963631),Xylopentaose (PubChem CID: 10146542),Xylose (PubChem CID: 644160),Xylotetraose (PubChem CID: 101601989),Xylotriose (PubChem CID: 12444993),pNP-α-l-arabinofuranoside (PubChem CID: 151437),pNP-β-d- cellobioside (PubChem CID: 165125),pNP-β-d-glucopyranoside (PubChem CID: 92930),pNP-β-d-xylopyranoside (PubChem CID: 91509)

          Comments

          Comment on this article