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      Monitoring Lens Transparency during in vitro Incubation by Scheimpflug Photography and Densitometric Image Analysis


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          Dragomirescu et al. [1983] described a 2-chamber flow system for the incubation of lenses with an inserted photometer to measure changes in lens transparency. Mester et al. [1984] applied the method to simulate conditions occurring during vitrectomy, which occasionally leads to reversible lens opacification. Using Scheimpflug photography (a modified SL 45 Topcon camera) instead of the transmission measurements of incubated lenses has the advantage that disorders in lens transparency can be exactly localized and the sensitivity is much higher than the photometer readings. Application of Scheimpflug photography required a modification of the incubation chamber. The application of the experimental set-up was demonstrated with pig lenses under various incubation conditions. The results obtained by densitometric image analysis of Scheimpflug photos were compared with transmission measurements by photometer.

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          Author and article information

          Ophthalmic Res
          Ophthalmic Research
          S. Karger AG
          04 December 2009
          : 17
          : 2
          : 96-101
          Department of Experimental Ophthalmology, University of Bonn, FRG
          265357 Ophthalmic Res 1985;17:96–101
          © 1985 S. Karger AG, Basel

          Copyright: All rights reserved. No part of this publication may be translated into other languages, reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying, recording, microcopying, or by any information storage and retrieval system, without permission in writing from the publisher. Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in government regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug. Disclaimer: The statements, opinions and data contained in this publication are solely those of the individual authors and contributors and not of the publishers and the editor(s). The appearance of advertisements or/and product references in the publication is not a warranty, endorsement, or approval of the products or services advertised or of their effectiveness, quality or safety. The publisher and the editor(s) disclaim responsibility for any injury to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements.

          : 25 January 1984
          : 16 May 1984
          Page count
          Pages: 6
          Original Paper

          Vision sciences,Ophthalmology & Optometry,Pathology
          Influence of incubation temperature,Incubation chamber,Lens transparency,in vitro incubation,Scheimpflug photography


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