The incidence of Campylobacter outbreaks caused by contaminated chicken livers has recently increased. We aimed to investigate where contamination occurs, and the drug resistance and genetic characteristics of Campylobacter from chicken livers sold in Beijing, China. The bacteria were isolated from 103 raw chicken livers bought from the retail market in two districts of Beijing. The E-test, Multilocus Sequence Typing (MLST) and pulsed-field gel electrophoresis techniques (PFGE) were used to study the antibiotic susceptibility and genetic sub-typing of the obtained isolates. A total of 42 Campylobacter isolates (30 C. jejuni and 12 C. coli) were obtained from 38 samples of the 103 samples tested (37%, 38/103). The rates of resistance against the tested antibiotics were as follows: erythromycin (2.38%, 1/42), azithromycin (4.76%, 2/42), streptomycin (4.76%, 2/40), gentamicin (40.47%, 17/42), chloramphenicol (11.90%, 5/42), ampicillin (23.81%, 10/42), nalidixic acid (92.85%, 39/42), ciprofloxacin (90.48%, 38/40), doxycycline (78.57%, 33/42), and tetracycline (83.33%, 35/42). The isolates were obtained both from the interior and exterior of the liver samples. Seven sequence types were identified among the 42 isolates; 23 PFGE patterns were found among 32 of the 42 isolates. Different PFGE patterns were identified in isolates from the interior and exterior of the same sample. In conclusion, both the interior and exterior of chicken livers can be contaminated with Campylobacter. They may therefore be a major food source of human campylobacteriosis in Beijing.