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      Regulation of Ornithine Decarboxylase in Rat Kidney

      ,

      Nephron

      S. Karger AG

      Kidney, Phorbol, Growth, Ornithine decarboxylase

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          Abstract

          Known stimulators of the calcium-sensitive phospholipid-dependent protein kinase C were investigated for their ability to regulate ornithine decarboxylase (ODC) activity in rat kidney. In the control state ODC activity averaged 84.9 ± 13.2 pmol mg<sup>-1</sup> min<sup>-1</sup> in a soluble fraction (n = 4). Four hours following the intraperitoneal injection of 2.5 nmol/g body weight of phorbol 12-myristate 13-acetate (PMA) activity increased to 284.1 ± 10.9 pmol mg<sup>1</sup> min<sup>-1</sup> (n = 4; p < 0.001). A chemically distinct stimulator of PKC, mezerein, had a similar effect on ODC in kidney and liver. Activity stimulated by PMA in kidney was dependent on the synthesis of both new mRNA and protein. Four hours following unilateral nephrectomy (UN<sub>X</sub>), ODC activity increased from 112.9 ± 15.6 pmol mg<sup>-1</sup> min<sup>-1</sup> in sham-operated animals to 319.1 ± 30.0 pmol mg<sup>-1</sup> min ‘ in animals postnephrectomy (n = 4; p < 0.01). Activity of ODC stimulated by phorbol esters was not additive to that seen following UN<sub>X</sub>. Twelve hours following the induction of renal growth by folic acid, ODC-specific activity was at basal levels. Neither UN<sub>X</sub> nor PMA were able to stimulate ODC at this time These data suggest that protein kinase C may be involved in the regulation of ODC in rat kidney.

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          Author and article information

          Journal
          NEF
          Nephron
          10.1159/issn.1660-8151
          Nephron
          S. Karger AG
          1660-8151
          2235-3186
          1990
          1990
          10 December 2008
          : 55
          : 4
          : 414-418
          Affiliations
          Division of Nephrology, Department of Internal Medicine, The University of Maryland Medical School, Baltimore, Md., USA
          Article
          186010 Nephron 1990;55:414–418
          10.1159/000186010
          2392194
          © 1990 S. Karger AG, Basel

          Copyright: All rights reserved. No part of this publication may be translated into other languages, reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying, recording, microcopying, or by any information storage and retrieval system, without permission in writing from the publisher. Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in government regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug. Disclaimer: The statements, opinions and data contained in this publication are solely those of the individual authors and contributors and not of the publishers and the editor(s). The appearance of advertisements or/and product references in the publication is not a warranty, endorsement, or approval of the products or services advertised or of their effectiveness, quality or safety. The publisher and the editor(s) disclaim responsibility for any injury to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements.

          Page count
          Pages: 5
          Categories
          Original Paper

          Cardiovascular Medicine, Nephrology

          Ornithine decarboxylase, Growth, Phorbol, Kidney

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