This study aimed to perform optometric analysis of the color space and contrast of electronic message. Four electronic messages with different color spaces and contrast were constructed. Color space was measured by “RGB Sliders” from the menu “Transparency” in the Microsoft Office PowerPoint 2011. The luminance was measured by the luminance meter. The reading speed was recorded as words per minute. Reading speed varies significantly in four different electronic messages with different color space and contrast ratio. The mean reading speed increased significantly from L4 to L3. The highest reading speed was found at the intermediate color space.