3
views
0
recommends
+1 Recommend
0 collections
    0
    shares
      • Record: found
      • Abstract: found
      • Article: found
      Is Open Access

      Enhanced cellulase production by decreasing intercellular pH through H +-ATPase gene deletion in Trichoderma reesei RUT-C30

      research-article

      Read this article at

      Bookmark
          There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.

          Abstract

          Background

          Cellulolytic enzymes produced by Trichoderma reesei are widely used for the industrial production of biofuels and chemicals from lignocellulose. We speculated that intracellular pH during the fermentation process can affect cellulase induction.

          Results

          In this study, two H +-ATPase genes, tre76238 and tre78757, were first identified in T. reesei. Deletion of tre76238 and tre78757 in T. reesei RUT-C30 confirmed that tre76238 has a major function in maintaining intracellular pH, whereas tre78757 has a minor function. The tre76238 deletion strain Δ76238 displayed a high level of cellulase production using cellulase-repressive glucose as a sole carbon source, along with intracellular acid accumulation and growth retardation. Our results indicated that intracellular acid accumulation in Δ76238 stimulated a significant increase in the cytosolic Ca 2+ levels. Ca 2+ channels were shown to be necessary for cellulase production using glucose as the carbon source in Δ76238. Delayed Δ76238 growth could be reversed by optimizing the medium’s nitrogen sources to produce ammonia for intracellular acid neutralization in the early phase. This may be useful for scale-up of cellulase production using glucose as the carbon source.

          Conclusions

          This study provides a new perspective for significant alterations in the cellulase expression pattern of T. reesei Δ76238, indicating a new mechanism for cellulase regulation under conditions of low intracellular pH.

          Electronic supplementary material

          The online version of this article (10.1186/s13068-019-1536-2) contains supplementary material, which is available to authorized users.

          Related collections

          Most cited references51

          • Record: found
          • Abstract: not found
          • Article: not found

          Measurement of cellulase activities

          T. Ghose (1987)
            Bookmark
            • Record: found
            • Abstract: found
            • Article: not found

            Sensors and regulators of intracellular pH.

            Protons dictate the charge and structure of macromolecules and are used as energy currency by eukaryotic cells. The unique function of individual organelles therefore depends on the establishment and stringent maintenance of a distinct pH. This, in turn, requires a means to sense the prevailing pH and to respond to deviations from the norm with effective mechanisms to transport, produce or consume proton equivalents. A dynamic, finely tuned balance between proton-extruding and proton-importing processes underlies pH homeostasis not only in the cytosol, but in other cellular compartments as well.
              Bookmark
              • Record: found
              • Abstract: found
              • Article: not found

              Genome sequencing and analysis of the biomass-degrading fungus Trichoderma reesei (syn. Hypocrea jecorina).

              Trichoderma reesei is the main industrial source of cellulases and hemicellulases used to depolymerize biomass to simple sugars that are converted to chemical intermediates and biofuels, such as ethanol. We assembled 89 scaffolds (sets of ordered and oriented contigs) to generate 34 Mbp of nearly contiguous T. reesei genome sequence comprising 9,129 predicted gene models. Unexpectedly, considering the industrial utility and effectiveness of the carbohydrate-active enzymes of T. reesei, its genome encodes fewer cellulases and hemicellulases than any other sequenced fungus able to hydrolyze plant cell wall polysaccharides. Many T. reesei genes encoding carbohydrate-active enzymes are distributed nonrandomly in clusters that lie between regions of synteny with other Sordariomycetes. Numerous genes encoding biosynthetic pathways for secondary metabolites may promote survival of T. reesei in its competitive soil habitat, but genome analysis provided little mechanistic insight into its extraordinary capacity for protein secretion. Our analysis, coupled with the genome sequence data, provides a roadmap for constructing enhanced T. reesei strains for industrial applications such as biofuel production.
                Bookmark

                Author and article information

                Contributors
                18801954428@163.com
                zhangoxu@qq.com
                yumengchen@163.com
                zhaojian@ecust.edu.cn
                +86-21-64253287 , wadexp@ecust.edu.cn
                weidzhi@163.com
                Journal
                Biotechnol Biofuels
                Biotechnol Biofuels
                Biotechnology for Biofuels
                BioMed Central (London )
                1754-6834
                13 August 2019
                13 August 2019
                2019
                : 12
                : 195
                Affiliations
                ISNI 0000 0001 2163 4895, GRID grid.28056.39, State Key Lab of Bioreactor Engineering, New World Institute of Biotechnology, , East China University of Science and Technology, ; P.O.B. 311, 130 Meilong Road, Shanghai, 200237 China
                Author information
                http://orcid.org/0000-0002-0440-0859
                Article
                1536
                10.1186/s13068-019-1536-2
                6691542
                17ed6732-5828-4cfb-a956-5acaa2337eb9
                © The Author(s) 2019

                Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License ( http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver ( http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

                History
                : 7 May 2019
                : 3 August 2019
                Funding
                Funded by: Open Funding Project of the State Key Laboratory of Bioreactor Engineering
                Funded by: Fundamental Research Funds for the Central Universities
                Award ID: 222201714053
                Award Recipient :
                Categories
                Research
                Custom metadata
                © The Author(s) 2019

                Biotechnology
                trichoderma reesei,glucose,h+-atpase,intercellular ph,ca2+ channels,calcium signaling
                Biotechnology
                trichoderma reesei, glucose, h+-atpase, intercellular ph, ca2+ channels, calcium signaling

                Comments

                Comment on this article