+1 Recommend
0 collections
      • Record: found
      • Abstract: found
      • Article: not found

      Identification of nitric oxide synthase as a protective locus against tuberculosis.

      Proceedings of the National Academy of Sciences of the United States of America

      pathology, Alleles, immunology, genetics, Tuberculosis, Polymorphism, Genetic, Polymerase Chain Reaction, deficiency, biosynthesis, Nitric Oxide Synthase, physiology, isolation & purification, Mycobacterium tuberculosis, Mice, Knockout, Mice, Inbred Strains, Mice, Inbred C57BL, Mice, Membrane Proteins, Male, microbiology, Lung, Isoenzymes, Immunosuppression, Immunity, Innate, Homozygote, Heterozygote, Haplotypes, pharmacology, Glucocorticoids, Genotype, Female, Exons, Disease Susceptibility, Crosses, Genetic, Cation Transport Proteins, Carrier Proteins, Animals

      Read this article at

          There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.


          Mutagenesis of the host immune system has helped identify response pathways necessary to combat tuberculosis. Several such pathways may function as activators of a common protective gene: inducible nitric oxide synthase (NOS2). Here we provide direct evidence for this gene controlling primary Mycobacterium tuberculosis infection using mice homozygous for a disrupted NOS2 allele. NOS2(-/-) mice proved highly susceptible, resembling wild-type littermates immunosuppressed by high-dose glucocorticoids, and allowed Mycobacterium tuberculosis to replicate faster in the lungs than reported for other gene-deficient hosts. Susceptibility appeared to be independent of the only known naturally inherited antimicrobial locus, NRAMP1. Progression of chronic tuberculosis in wild-type mice was accelerated by specifically inhibiting NOS2 via administration of N6-(1-iminoethyl)-L-lysine. Together these findings identify NOS2 as a critical host gene for tuberculostasis.

          Related collections

          Author and article information



          Comment on this article