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      Beclin-phosphatidylinositol 3-kinase complex functions at the trans-Golgi network.

      1 , , ,
      EMBO reports
      Oxford University Press (OUP)

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          Abstract

          Autophagy is an intracellular bulk protein degradation system. Beclin is known to be involved in this process; however, its role is unclear. In this study, we showed that Beclin was co-immunoprecipitated with phosphatidylinositol (PtdIns) 3-kinase, which is also required for autophagy, suggesting that Beclin is a component of the PtdIns 3-kinase complex. Quantitative analyses using a cross-linker showed that all Beclin forms a complex with PtdIns 3-kinase, whereas approximately 50% of PtdIns 3-kinase remains free from Beclin. Indirect immunofluorescence microscopy demonstrated that the majority of Beclin and PtdIns 3-kinase localize to the trans-Golgi network (TGN). Some PtdIns 3-kinase is also distributed in the late endosome. These results suggest that Beclin and PtdIns 3-kinase control autophagy as a complex at the TGN.

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          Author and article information

          Journal
          EMBO Rep
          EMBO reports
          Oxford University Press (OUP)
          1469-221X
          1469-221X
          Apr 2001
          : 2
          : 4
          Affiliations
          [1 ] Department of Cell Biology, National Institute for Basic Biology, Nishigonaka 38, Myodaiji-cho, Okazaki 444-8585, Japan.
          Article
          2/4/330
          10.1093/embo-reports/kve061
          1083858
          11306555
          188ebd34-ea6c-49d3-8f47-910f53c6543e
          History

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