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      Preparative separation of two alkaloids from devil pepper radix ( Rauvolfia verticillata [Lour.] Baill.) by ph-zone-refining counter-current chromatography


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          A method was developed for the preparative separation of two alkaloids from the crude extract of the radix of Rauvolfia verticillata (Lour.) Baill. in a single run. The two-phase solvent system composed of petroleum ether–ethyl acetate–methanol–water (5:5:2:8, v/v), where triethylamine (40 mmol/L) was added to the upper organic phase as the stationary phase and hydrochloric acid (10 mmol/L) was added to the lower aqueous phase as the mobile phase, was selected for this separation by pH-zone-refining counter-current chromatography (PZRCCC). For the preparative separation, the apparatus was rotated at a speed 850 rpm, while the mobile phase was pumped into the column at 2 mL/min. As a result, 112 mg of reserpine and 21 mg of yohimbine were obtained from 3 g of crude extract in a single run. The analysis of the isolated compounds was determined by high-performance liquid chromatography (HPLC) at 230 nm with purities of over 91.0%, and the chemical identification was carried out by the data of electrospray ionization–mass spectrometry (ESI–MS) and nuclear magnetic resonance (NMR) spectroscopy. The technique introduced in this paper is an efficient method for preparative separation of reserpine and yohimbine from devil pepper radix. It will be beneficial to utilize medicinal materials and also useful for the separation, purification, and pharmacological study of Chinese herbal ingredients.

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          Author and article information

          Acta Chromatographica
          Acta Chromatographica
          Akadémiai Kiadó
          June 2018
          : 30
          : 2
          : 81-84
          [ 1 ] Shandong Key Laboratory of TCM Quality Control Technology, Shandong Analysis and Test Center, Shandong Academy of Sciences , China
          [ 2 ] College of Food Science and Engineering, Shandong Agricultural University , Taian, 271018, China
          [ 3 ]National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences , Beijing, 100700, China
          Author notes

          Author for correspondence: wxjn1998@ 123456126.com

          © 2017 The Author(s)

          This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (https://creativecommons.org/licenses/by-nc/4.0/), which permits unrestricted use, distribution, and reproduction in any medium for non-commercialpurposes, provided the original author and source are credited, a link to the CC License is provided, and changes - if any - are indicated.

          : 3 March 2017
          : 17 April 2017
          Page count
          Pages: 4
          Original Research Paper

          Materials properties,Nanomaterials,Chemistry,Nanotechnology,Analytical chemistry,Thin films & surfaces
          pH-zone-refining counter-current chromatography,yohimbine, Rauvolfia verticillata (Lour.) Baill.,alkaloids,reserpine


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