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      Interaction of Immune Complexes with Cultured Rabbit Glomerular Cells

      Nephron

      S. Karger AG

      Glomerular cell culture, Immune complexes, Fibronectin

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          Abstract

          Rabbit glomerular cell cultures were established from adolescent and mature rabbits in the absence of antibiotics. Fibroblast growth factor was added to half the cultures. Immune complexes (IC) formed with 125I bovine albumin and rabbit antibody were incubated with 10-day-old cultures for 44 h. Cell-IC interaction was observed in all samples but was increased in cultures without growth factor: the effect was not age dependent. Cells cultured without growth factor had increased surface accumulation of fibronectin. Addition of antifibronectin antibody to cell cultures did not inhibit cell complex interaction. The presence of unlabeled IC reduced labeled IC binding in cultures without growth factor. Binding of IC made with F(ab’)2 fragments exceeded that of IC made with intact IgG. The results suggest IC bind with cultured glomerular cells and the degree of interaction is influenced by the presence of growth factors which alter cell membrane composition.

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          Author and article information

          Journal
          NEF
          Nephron
          10.1159/issn.1660-8151
          Nephron
          S. Karger AG
          1660-8151
          2235-3186
          1982
          1982
          03 December 2008
          : 32
          : 4
          : 351-358
          Affiliations
          Department of Medicine, Clowes Research Laboratory, Indiana University School of Medicine, Indianapolis, Ind., USA
          Article
          182878 Nephron 1982;32:351–358
          10.1159/000182878
          6220228
          © 1982 S. Karger AG, Basel

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          Page count
          Pages: 8
          Categories
          Original Paper

          Cardiovascular Medicine, Nephrology

          Glomerular cell culture, Immune complexes, Fibronectin

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