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Agrobacterium tumefaciens is the preferred method for transformation of a wide range
of plant species. Commonly, the genes to be transferred are cloned between the left
and right T-DNA borders of so-called binary T-DNA vectors that can replicate both
in E. coli and Agrobacterium. Because these vectors are generally large, cloning can
be time-consuming and laborious. Recently, the GATEWAY conversion technology has provided
a fast and reliable alternative to the cloning of sequences into large acceptor plasmids.