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      GATEWAY™ vectors for Agrobacterium-mediated plant transformation

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      Trends in Plant Science

      Elsevier BV

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          Abstract

          Agrobacterium tumefaciens is the preferred method for transformation of a wide range of plant species. Commonly, the genes to be transferred are cloned between the left and right T-DNA borders of so-called binary T-DNA vectors that can replicate both in E. coli and Agrobacterium. Because these vectors are generally large, cloning can be time-consuming and laborious. Recently, the GATEWAY conversion technology has provided a fast and reliable alternative to the cloning of sequences into large acceptor plasmids.

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          Author and article information

          Journal
          Trends in Plant Science
          Trends in Plant Science
          Elsevier BV
          13601385
          May 2002
          May 2002
          : 7
          : 5
          : 193-195
          Article
          10.1016/S1360-1385(02)02251-3
          11992820
          © 2002

          https://www.elsevier.com/tdm/userlicense/1.0/

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