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      Probing the structure of RNAs in solution.

      Nucleic Acids Research
      Base Sequence, DNA, analysis, Molecular Sequence Data, Nucleic Acid Conformation, RNA, genetics, Ribonucleases

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          Abstract

          During these last years, a powerful methodology has been developed to study the secondary and tertiary structure of RNA molecules either free or engaged in complex with proteins. This method allows to test the reactivity of every nucleotide towards chemical or enzymatic probes. The detection of the modified nucleotides and RNase cleavages can be conducted by two different paths which are oriented both by the length of the studied RNA and by the nature of the probes used. The first one uses end-labeled RNA molecule and allows to detect only scissions in the RNA chain. The second approach is based on primer extension by reverse transcriptase and detects stops of transcription at modified or cleaved nucleotides. The synthesized cDNA fragments are then sized by electrophoresis on polyacrylamide:urea gels. In this paper, the various structure probes used so far are described, and their utilization is discussed.

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          Author and article information

          Journal
          2446263
          306456
          10.1093/nar/15.22.9109

          Chemistry
          Base Sequence,DNA,analysis,Molecular Sequence Data,Nucleic Acid Conformation,RNA,genetics,Ribonucleases

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