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      Diagnosis of antigenic markers of acute toxoplasmosis by IgG avidity immunoblotting Translated title: Diagnostic des marqueurs antigéniques de toxoplasmose aigue par immunoréplique d’avidité des IgG

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          Abstract

          To perform IgG avidity immunoblotting assay for detection of acute toxoplasmosis, 100 serum samples were collected from Tehran, Iran. The presence of Toxoplasma-specific IgG and IgM antibodies were checked by commercial Trinity kit. Samples were categorized in acute and chronic phases of Toxoplasma gondii infection according to IgG avidity ELISA. IgG avidity immunoblotting was performed, and antigenic bands with molecular weights of 22, 25, 28, 30, 32, 42, 44, 49, 55, 60, 66, 69, 88, 106, 130 and 157 kDa were recognized as low avidity markers. The most prevalent antigen for low avidity was p22. It is concluded that IgG avidity immunoblotting could distinguish acute and chronic phases of T. gondii infection.

          Translated abstract

          Pour exécuter un essai d’immunoréplique d’avidité des IgG pour la détection de la toxoplasmose aigue, cent échantillons de sérums ont été collectés à Téhéran, Iran. La présence d’anticorps IgG et IgM spécifiques de Toxoplasma a été vérifiée par le kit commercial Trinity. Les échantillons ont été caractérisés comme phases chronique ou aigue d’infection par Toxoplasma gondii en fonction d’avidité ELISA des IgG. Une immunoréplique d’avidité des IgG a été effectuée, et les bandes antigéniques de poids moléculaires de 22, 25, 28, 30, 44, 49, 55, 60, 66, 69, 88, 106, 130 et 157 kDa ont été reconnues comme des marqueurs de basse avidité. L’antigène le plus prévalent pour la basse avidité était p22. On conclut que l’immunoréplique d’avidité des IgG pourrait distinguer les phases chroniques ou aigue d’infection par T. gondii.

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          Toxoplasmosis: A history of clinical observations.

          It has been 100 years since Toxoplasma gondii was initially described in Tunis by Nicolle and Manceaux (1908) in the tissues of the gundi (Ctenodoactylus gundi) and in Brazil by Splendore (1908) in the tissues of a rabbit. Toxoplasma gondii is a ubiquitous, Apicomplexan parasite of warm-blooded animals that can cause several clinical syndromes including encephalitis, chorioretinitis, congenital infection and neonatal mortality. Fifteen years after the description of T. gondii by Nicolle and Manceaux a fatal case of toxoplasmosis in a child was reported by Janků. In 1939 Wolf, Cowen and Paige were the first to conclusively identify T. gondii as a cause of human disease. This review examines the clinical manifestations of infection with T. gondii and the history of the discovery of these manifestations.
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            Recent developments for diagnosis of toxoplasmosis.

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              Effect of testing for IgG avidity in the diagnosis of Toxoplasma gondii infection in pregnant women: experience in a US reference laboratory.

              The usefulness of testing for IgG avidity in association with Toxoplasma gondii was evaluated in a US reference laboratory. European investigators have reported that high-avidity IgG toxoplasma antibodies exclude acute infection in the preceding 3 months. In this US study, 125 serum samples taken from 125 pregnant women in the first trimester were chosen retrospectively, because either the IgM or differential agglutination (AC/HS) test in the Toxoplasma serologic profile suggested or was equivocal for a recently acquired infection. Of 93 (74.4%) serum samples with either positive or equivocal results in the IgM ELISA, 52 (55.9%) had high-avidity antibodies, which suggests that the infection probably was acquired before gestation. Of 87 (69.6%) serum samples with an acute or equivocal result in the AC/HS test, 35 (40.2%) had high-avidity antibodies. Forty women were given spiramycin, to prevent congenital transmission, and 7 (17.5%) had high-avidity antibodies. These findings highlight the value of testing a single serum sample obtained in the first trimester of pregnancy for IgG avidity.
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                Author and article information

                Journal
                Parasite
                Parasite
                parasite
                Parasite
                EDP Sciences
                1252-607X
                1776-1042
                2013
                22 May 2013
                : 20
                : ( publisher-idID: parasite/2013/01 )
                Affiliations
                School of Public Health, Tehran University of Medical Sciences Tehran Iran
                Author notes
                Article
                parasite120014 10.1051/parasite/2013017
                10.1051/parasite/2013017
                3718541
                23688778
                © S. Ali-Heydari et al., published by EDP Sciences, 2013

                This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

                Page count
                Figures: 3, Tables: 0, Equations: 0, References: 14, Pages: 4
                Categories
                Research Article

                avidity immunoblotting, toxoplasmosis

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