Phosphoproteomic analyses of kidneys of Atlantic salmon infected with Aeromonas salmonicida

Abstract

Aeromonas salmonicida ( A. salmonicida) is a pathogenic bacterium that causes furunculosis and poses a significant global risk, particularly in economic activities such as Atlantic salmon (Salmo salar) farming. In a previous study, we identified proteins that are significantly upregulated in kidneys of Atlantic salmon challenged with A. salmonicida. Phosphoproteomic analyses were conducted to further clarify the dynamic changes in protein phosphorylation patterns triggered by bacterial infection. To our knowledge, this is the first study to characterize phosphorylation events in proteins from A. salmonicida-infected Atlantic salmon. Overall, we identified over 5635 phosphorylation sites in 3112 proteins, and 1502 up-regulated and 77 down-regulated proteins quantified as a 1.5-fold or greater change relative to control levels. Based on the combined data from proteomic and motif analyses, we hypothesize that five prospective novel kinases (VRK3, GAK, HCK, PKCδ and RSK6) with common functions in inflammatory processes and cellular pathways to regulate apoptosis and the cytoskeleton could serve as potential biomarkers against bacterial propagation in fish. Data from STRING-based functional network analyses indicate that fga is the most central protein. Our collective findings provide new insights into protein phosphorylation patterns, which may serve as effective indicators of A. salmonicida infection in Atlantic salmon.

Contributors

Dong Yang: [e-mail]

Ying Liu: [e-mail]

Journal

Journal ID (nlm-ta): Sci Rep

Journal ID (iso-abbrev): Sci Rep

Title: Scientific Reports

Publisher: Nature Publishing Group UK (London )

ISSN (Electronic): 2045-2322

Publication date (Electronic): 14 February 2019

Publication date PMC-release: 14 February 2019

Publication date Collection: 2019

Volume: 9

Affiliations

[1 ]ISNI 0000 0004 1792 5587, GRID grid.454850.8, Key Laboratory of Experimental Marine Biology, , Institute of Oceanology, Chinese Academy of Sciences, ; Qingdao, 266071 China

[2 ]ISNI 0000 0004 5998 3072, GRID grid.484590.4, Laboratory for Marine Fisheries Science and Food Production Processes, , Qingdao National Laboratory for Marine Science and Technology, ; Qingdao, 266235 China

[3 ]ISNI 0000 0004 1797 8419, GRID grid.410726.6, University of Chinese Academy of Sciences, ; Beijing, 100039 China

[4 ]ISNI 0000 0001 1867 7333, GRID grid.410631.1, Dalian Ocean University, ; Dalian, P. R. China

[5 ]ISNI 0000 0004 0457 9072, GRID grid.419611.a, State Key Laboratory of Proteomics, , Beijing Proteome Research Center, National Center for Protein Sciences (Beijing), Beijing Institute of Lifeomics, ; Beijing, 102206 P. R. China

Article

Publisher ID: 38890

DOI: 10.1038/s41598-019-38890-3

PMC ID: 6376026

PubMed ID: 30765835

Copyright statement: © The Author(s) 2019

License:

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

Categories

Subject: Article

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