Geographical distribution of Amblyomma cajennense ( sensu lato) ticks (Parasitiformes: Ixodidae) in Brazil, with description of the nymph of A. cajennense ( sensu stricto)

Abstract

Background

Until recently, Amblyomma cajennense (Fabricius, 1787) was considered to represent a single tick species in the New World. Recent studies have split this taxon into six species. While the A. cajennense species complex or A. cajennense ( sensu lato) ( s.l.) is currently represented by two species in Brazil, A. cajennense ( sensu stricto) ( s.s.) and Amblyomma sculptum Berlese, 1888 , their geographical distribution is poorly known .

Methods

The distribution of the A. cajennense ( s.l.) in Brazil was determined by morphological examination of all lots of A. cajennense ( s.l.) in two large tick collections of Brazil, and by collecting new material during three field expeditions in the possible transition areas between the distribution ranges of A. cajennense ( s.s.) and A. sculptum. Phylogenetic analysis inferred from the ITS2 rRNA gene was used to validate morphological results. Morphological description of the nymphal stage of A. cajennense ( s.s.) is provided based on laboratory-reared specimens.

Results

From the tick collections, a total 12,512 adult ticks were examined and identified as 312 A. cajennense ( s.s.), 6,252 A. sculptum and 5,948 A. cajennense ( s.l.). A total of 1,746 ticks from 77 localities were collected during field expeditions, and were identified as 249 A. cajennense ( s.s.), 443 A. sculptum, and 1,054 A. cajennense ( s.l.) [these A. cajennense ( s.l.) ticks were considered to be males of either A. cajennense ( s.s.) or A. sculptum]. At least 23 localities contained the presence of both A. cajennense ( s.s.) and A. sculptum in sympatry. DNA sequences of the ITS2 gene of 50 ticks from 30 localities confirmed the results of the morphological analyses. The nymph of A. cajennense ( s.s.) is morphologically very similar to A. sculptum.

Conclusion

Our results confirmed that A. cajennense ( s.l.) is currently represented in Brazil by only two species, A. cajennense ( s.s.) and A. sculptum. While these species have distinct distribution areas in the country, they are found in sympatry in some transition areas. The current distribution of A. cajennense ( s.l.) has important implications to public health, since in Brazil A. sculptum is the most important vector of the bacterium Rickettsia rickettsii, the etiological agent of Brazilian spotted fever.

Electronic supplementary material

The online version of this article (doi:10.1186/s13071-016-1460-2) contains supplementary material, which is available to authorized users.