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      Comparison of multiple methods for quantification of microbial biofilms grown in microtiter plates.

      Journal of Microbiological Methods

      metabolism, Xanthenes, Tetrazolium Salts, Staining and Labeling, Oxazines, Organic Chemicals, analogs & derivatives, Methylene Blue, physiology, Gram-Positive Bacteria, Gram-Negative Aerobic Rods and Cocci, Gentian Violet, Fluoresceins, Colony Count, Microbial, Candida albicans, growth & development, Biofilms

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          Abstract

          In the present study six assays for the quantification of biofilms formed in 96-well microtiter plates were optimised and evaluated: the crystal violet (CV) assay, the Syto9 assay, the fluorescein diacetate (FDA) assay, the resazurin assay, the XTT assay and the dimethyl methylene blue (DMMB) assay. Pseudomonas aeruginosa, Burkholderia cenocepacia, Staphylococcus aureus, Propionibacterium acnes and Candida albicans were used as test organisms. In general, these assays showed a broad applicability and a high repeatability for most isolates. In addition, the estimated numbers of CFUs present in the biofilms show limited variations between the different assays. Nevertheless, our data show that some assays are less suitable for the quantification of biofilms of particular isolates (e.g. the CV assay for P. aeruginosa).

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          Journal
          10.1016/j.mimet.2007.11.010
          18155789

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