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      Ecophysiology of Defluviicoccus-related tetrad-forming organisms in an anaerobic-aerobic activated sludge process.

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      Environmental microbiology

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          Abstract

          A group of uncultured tetrad-forming organisms (TFOs) was enriched in an acetate-fed anaerobic-aerobic sequencing membrane bioreactor showing deteriorated enhanced biological phosphorus removal capacity. Based on 16S rRNA gene clone library and fluorescence in situ hybridization (FISH) analyses, these TFOs were identified as novel members of the Defluviicoccus cluster in the Alphaproteobacteria, accounting for 90 +/- 5% of the EUBmix FISH-detectable bacterial cell area in the reactor biomass. Microautoradiography in combination with FISH and polyhydroxyalkanoate (PHA) staining revealed that these Defluviicoccus-related TFOs could take up and transform acetate, lactate, propionate and pyruvate, but not aspartic acid and glucose, into PHA under anaerobic conditions. In contrast, under continuous anaerobic-aerobic cultivation, Defluviicoccus vanus, the only cultured strain from the cluster, was able to take up glucose with concurrent glycogen consumption and PHA production under anaerobic conditions. Under subsequent aerobic conditions, the accumulated PHA was utilized and the biomass glycogen levels were restored. These findings not only re-confirmed these Defluviicoccus-related TFOs as glycogen-accumulating organisms, but also revealed unexpected levels of physiological, phylogenetic and morphological diversity among members of the Defluviicoccus cluster.

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          Author and article information

          Journal
          Environ. Microbiol.
          Environmental microbiology
          1462-2912
          1462-2912
          Jun 2007
          : 9
          : 6
          Affiliations
          [1 ] Division of Environmental Science and Engineering, National University of Singapore, Singapore 117576.
          Article
          EMI1267
          10.1111/j.1462-2920.2007.01267.x
          17504486
          1aa8dc01-82bd-4bd0-989e-3de0d069a28c
          History

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