Synthesis and immunogenicity of PG-tb1 monovalent glycoconjugate.

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Abstract

A PG-tb1 hapten from the West Beijing strains of Mycobacterium tuberculosis cell wall has been efficiently synthesized and conjugated to CRM197 in a simple way as linker-equipped carbohydrate by applying squaric acid chemistry for an original neoglycoprotein, creating a potent T-dependent conjugate vaccine. The intermediate monoester can be easily purified and the degree of incorporation can be monitored by MALDI-TOF mass spectrometry. After administered systemically in mice without any adjuvant, the conjugate induced high antigen-specific IgG levels in serum. Furthermore, following the third immunization, significant antibody titers frequently exceeding 0.8 million were observed in the sera of mice vaccinated with PG-CRM197 conjugate which showed the potential for preparation of TB vaccine.

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Journal

Journal ID (iso-abbrev): Eur J Med Chem

Title: European journal of medicinal chemistry

Publisher: Elsevier BV

ISSN (Electronic): 1768-3254

ISSN (Print): 0223-5234

Publication date (Electronic): Jul 07 2017

Volume: 134

Affiliations

[1 ] China International Science and Technology Cooperation Base of Food Nutrition/Safety and Medicinal Chemistry, Sino-French Joint Lab of Food Nutrition/Safety and Medicinal Chemistry, Key Laboratory of Industrial Fermentation Microbiology of Ministry of Education, Tianjin Key Laboratory of Industry Microbiology, College of Biotechnology, Tianjin University of Science and Technology, Tianjin 300457, China.

[2 ] Affiliated Hospital of Logistic University of Chinese People's Armed Police Force (PAPF), Tianjin 300162, China.

[3 ] Key Laboratory of Food Nutrition and Safety of Ministry of Education, College of Food Engineering and Biotechnology, Tianjin University of Science and Technology, Tianjin 300457, China.

[4 ] China International Science and Technology Cooperation Base of Food Nutrition/Safety and Medicinal Chemistry, Sino-French Joint Lab of Food Nutrition/Safety and Medicinal Chemistry, Key Laboratory of Industrial Fermentation Microbiology of Ministry of Education, Tianjin Key Laboratory of Industry Microbiology, College of Biotechnology, Tianjin University of Science and Technology, Tianjin 300457, China. Electronic address: yyang@tust.edu.cn.

[5 ] China International Science and Technology Cooperation Base of Food Nutrition/Safety and Medicinal Chemistry, Sino-French Joint Lab of Food Nutrition/Safety and Medicinal Chemistry, Key Laboratory of Industrial Fermentation Microbiology of Ministry of Education, Tianjin Key Laboratory of Industry Microbiology, College of Biotechnology, Tianjin University of Science and Technology, Tianjin 300457, China. Electronic address: yupeng@tust.edu.cn.

[6 ] CanSino Biologics Inc., Tianjin Enterprise Key Laboratory of Respiratory Bacterial Recombination and Conjugated Vaccine, Tianjin 300457, China. Electronic address: tao.zhu@cansinotech.com.

Article

Publisher Item ID: S0223-5234(17)30224-6

DOI: 10.1016/j.ejmech.2017.03.058

PubMed ID: 28411454

SO-VID: 1b5cb6cc-cf3b-4b69-a4db-ad7be8e1d99e

History

Keywords: Bioconjugation,Carbohydrate,Glycoconjugate,Synthesis,Vaccine

Data availability:

Keywords: Bioconjugation, Carbohydrate, Glycoconjugate, Synthesis, Vaccine

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