Effects of an Endogenous Cyclic AMP-Dependent Protein Kinase Catalytic Subunit on Ca-Uptake by Plasma Membrane Vesicles from Rat Mesenteric Artery

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Abstract

A plasma membrane-enriched fraction of vesicles from rat mesenteric artery possessed ATP-dependent calcium properties similar to those which have been reported previously. The catalytic subunit from a soluble, cyclic AMP-dependent protein kinase was prepared from the same tissue, in partially purified form. Calcium uptake by membrane vesicles increased 30% in the presence of this kinase. This effect was proportional to the kinase concentration, and was blocked by either catalytic subunit inhibitor or by prior boiling of the subunit preparation. Stimulation of calcium uptake by the kinase occurred maximally between 1 and 10 µ Mfree calcium. These findings suggest that cyclic AMP and its dependent protein kinase may participate in the regulation of smooth muscle tension at high free myoplasmic calcium concentrations.

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Journal

Journal ID (publisher-id): JVR

Journal ID (nlm-ta): J Vasc Res

Journal ID (doi): 10.1159/issn.1018-1172

Title: Journal of Vascular Research

Publisher: S. Karger AG

ISSN (Print): 1018-1172

ISSN (Electronic): 1423-0135

Publication date Subscription-year: 1984

Publication date (Print): 1984

Publication date (Electronic): 23 September 2008

Volume: 21

Issue: 6

Pages: 257-266

Affiliations

Department of Neurosciences, McMaster University, Faculty of Health Sciences, Hamilton, Canada

Article

Publisher ID: 158528 Other ID: Blood Vessels 1984;21:257–266

DOI: 10.1159/000158528

SO-VID: 1c46dd92-6026-45c9-bc10-580c0f58fc76

License:

Copyright: All rights reserved. No part of this publication may be translated into other languages, reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying, recording, microcopying, or by any information storage and retrieval system, without permission in writing from the publisher. Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in government regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug. Disclaimer: The statements, opinions and data contained in this publication are solely those of the individual authors and contributors and not of the publishers and the editor(s). The appearance of advertisements or/and product references in the publication is not a warranty, endorsement, or approval of the products or services advertised or of their effectiveness, quality or safety. The publisher and the editor(s) disclaim responsibility for any injury to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements.

History

Date received : 07 October 1983

Date accepted : 02 March 1984

Page count

Pages: 10

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