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      Effects of an Endogenous Cyclic AMP-Dependent Protein Kinase Catalytic Subunit on Ca-Uptake by Plasma Membrane Vesicles from Rat Mesenteric Artery

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      Journal of Vascular Research

      S. Karger AG

      Protein kinase, Calcium uptake, Smooth muscle

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          Abstract

          A plasma membrane-enriched fraction of vesicles from rat mesenteric artery possessed ATP-dependent calcium properties similar to those which have been reported previously. The catalytic subunit from a soluble, cyclic AMP-dependent protein kinase was prepared from the same tissue, in partially purified form. Calcium uptake by membrane vesicles increased 30% in the presence of this kinase. This effect was proportional to the kinase concentration, and was blocked by either catalytic subunit inhibitor or by prior boiling of the subunit preparation. Stimulation of calcium uptake by the kinase occurred maximally between 1 and 10 µ Mfree calcium. These findings suggest that cyclic AMP and its dependent protein kinase may participate in the regulation of smooth muscle tension at high free myoplasmic calcium concentrations.

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          Author and article information

          Journal
          JVR
          J Vasc Res
          10.1159/issn.1018-1172
          Journal of Vascular Research
          S. Karger AG
          1018-1172
          1423-0135
          1984
          1984
          23 September 2008
          : 21
          : 6
          : 257-266
          Affiliations
          Department of Neurosciences, McMaster University, Faculty of Health Sciences, Hamilton, Canada
          Article
          158528 Blood Vessels 1984;21:257–266
          10.1159/000158528
          © 1984 S. Karger AG, Basel

          Copyright: All rights reserved. No part of this publication may be translated into other languages, reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying, recording, microcopying, or by any information storage and retrieval system, without permission in writing from the publisher. Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in government regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug. Disclaimer: The statements, opinions and data contained in this publication are solely those of the individual authors and contributors and not of the publishers and the editor(s). The appearance of advertisements or/and product references in the publication is not a warranty, endorsement, or approval of the products or services advertised or of their effectiveness, quality or safety. The publisher and the editor(s) disclaim responsibility for any injury to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements.

          Page count
          Pages: 10
          Categories
          Research Paper

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