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      Western blotting.

      Methods (San Diego, Calif.)

      Animals, Antigens, chemistry, Biotinylation, Blotting, Western, instrumentation, methods, Collodion, Diffusion, Electrophoresis, Electrophoresis, Polyacrylamide Gel, Humans, Immunoblotting, Membranes, Artificial, Proteins, analysis, Solvents

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          Abstract

          Western blotting (protein blotting or immunoblotting) is a powerful and important procedure for the immunodetection of proteins post-electrophoresis, particularly proteins that are of low abundance. Since the inception of the protocol for protein transfer from an electrophoresed gel to a membrane in 1979, protein blotting has evolved greatly. The scientific community is now confronted with a variety of ways and means to carry out this transfer. This review describes the various procedures that have been used to transfer proteins from a gel to a membrane based on the principles of simple diffusion, vacuum-assisted solvent flow and electrophoretic elution. Finally, a brief description of methods generally used to detect antigens on blots is also described.

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          Author and article information

          Journal
          16483794
          10.1016/j.ymeth.2005.11.007

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