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      NanoPack: visualizing and processing long-read sequencing data

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          Abstract

          Summary

          Here we describe NanoPack, a set of tools developed for visualization and processing of long-read sequencing data from Oxford Nanopore Technologies and Pacific Biosciences.

          Availability and implementation

          The NanoPack tools are written in Python3 and released under the GNU GPL3.0 License. The source code can be found at https://github.com/wdecoster/nanopack, together with links to separate scripts and their documentation. The scripts are compatible with Linux, Mac OS and the MS Windows 10 subsystem for Linux and are available as a graphical user interface, a web service at http://nanoplot.bioinf.be and command line tools.

          Supplementary information

          Supplementary data are available at Bioinformatics online.

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          Most cited references11

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          The Sequence Alignment/Map format and SAMtools

          Summary: The Sequence Alignment/Map (SAM) format is a generic alignment format for storing read alignments against reference sequences, supporting short and long reads (up to 128 Mbp) produced by different sequencing platforms. It is flexible in style, compact in size, efficient in random access and is the format in which alignments from the 1000 Genomes Project are released. SAMtools implements various utilities for post-processing alignments in the SAM format, such as indexing, variant caller and alignment viewer, and thus provides universal tools for processing read alignments. Availability: http://samtools.sourceforge.net Contact: rd@sanger.ac.uk
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            Matplotlib: A 2D Graphics Environment

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              The NumPy Array: A Structure for Efficient Numerical Computation

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                Author and article information

                Contributors
                Role: Associate Editor
                Journal
                Bioinformatics
                Bioinformatics
                bioinformatics
                Bioinformatics
                Oxford University Press
                1367-4803
                1367-4811
                01 August 2018
                14 March 2018
                14 March 2018
                : 34
                : 15
                : 2666-2669
                Affiliations
                [1 ]Neurodegenerative Brain Diseases Group, VIB & University of Antwerp, Antwerp, Belgium
                [2 ]Bioinformatics, Neuromics Support Facility, Center for Molecular Neurology, VIB & University of Antwerp, Antwerp, Belgium
                [3 ]Department of Biomolecular Engineering and Bioinformatics, University of California Santa Cruz, Santa Cruz, CA, USA
                Author notes
                To whom correspondence should be addressed. Email: wouter.decoster@ 123456molgen.vib-ua.be
                Author information
                http://orcid.org/0000-0002-5248-8197
                Article
                bty149
                10.1093/bioinformatics/bty149
                6061794
                29547981
                1c654121-4b58-42a6-9b85-ba781fb58a0a
                © The Author(s) 2018. Published by Oxford University Press.

                This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

                History
                : 02 January 2018
                : 19 February 2018
                : 12 March 2018
                Page count
                Pages: 4
                Funding
                Funded by: VIB 10.13039/501100004727
                Funded by: Flanders Institute for Biotechnology, Belgium
                Funded by: University of Antwerp 10.13039/501100007660
                Funded by: Flanders Agency for Innovation and Entrepreneurship
                Funded by: NSF 10.13039/100000001
                Funded by: DGE 10.13039/100000082
                Award ID: 1339067
                Categories
                Applications Notes
                Sequence Analysis

                Bioinformatics & Computational biology
                Bioinformatics & Computational biology

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