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      AB228. Research on the mechanism of androgen replacement therapy improving erectile dysfunction in castrated rats

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          Abstract

          Objective

          To investigate the mechanism of androgen replacement therapy (ART) improving erectile dysfunction (ED) in castrated rats.

          Methods

          We randomly divided 40 8-week-old healthy male SD rats into 4 groups: group A was the control, and rats of the group B, C and D were castrated, then rats in the groups C and D were treated with different concentrations of testosterone undecanoate orally every day (C: 10 mg/kg, D: 20 mg/kg), while other groups with 0.9% NS instead. 8weeks’ treatment later, we determined the level of serum testosterone and assessed the erectile function of rats. Western blot, immunohistochemistry were performed to detect the level of target proteins.

          Results

          (I) The level of serum testosterone and erectile function (Max ICP/MAP): group Bwas significantly lower than group A, C and D, and group D was higher compared with group C; (II) effect of castration and ART on endothelial cells and androgen receptor (AR)/vascular endothelial growth factor (VEGF)/cyclin A pathway: the expression of CD31, vWF and AR/VEGF/cyclin A in group B were lower than group A, C and D, and group D was higher compared with group C; (III) effect of castration and ART on corpus cavernosum smooth muscle cells (CCSMCs) and TGF-β/S1P2/RhoA/ROCK pathway: the expression of α-sma in group B were lower than group A, C and D, and group D was higher compared with group C; while the expression of TGF-β/S1P2/RhoA/ROCK1 were higher in group B than group A, C and D, and group D was lower compared with group C.

          Conclusions

          ART can improve ED in castrated rats through promoting the proliferation of corpus cavernosum endothelial cells by activating AR/VEGF/cyclin A pathway; decreasing the contraction of CCSMCs and corporal fibrosis by inhibiting TGF-β/S1P2/RhoA/ROCK pathway, which provides reference for revealing the mechanism of ART treating ED associated late-onset hypogonadism.

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          Author and article information

          Journal
          Transl Androl Urol
          Transl Androl Urol
          TAU
          Translational Andrology and Urology
          AME Publishing Company
          2223-4691
          April 2016
          April 2016
          : 5
          : Suppl 1
          : AB228
          Affiliations
          [1]Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China
          Article
          tau-05-S1-AB228
          10.21037/tau.2016.s228
          4842567
          1cd10fe3-081f-47a2-bcf6-096bd70f2856
          2016 Translational Andrology and Urology. All rights reserved.
          History
          Categories
          Printed Abstracts

          androgen,erectile dysfunction (ed),castration
          androgen, erectile dysfunction (ed), castration

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