A small change in the environment (a prepulse) that just precedes a startle-eliciting stimulus can reduce the size of the elicited reflex, but a prepulse does not appear to diminish the ability of the startle-eliciting stimulus to depress a startle response elicited a little later. The reflex-eliciting and reflex-modifying effects of startle stimuli seem to be independent. However, most support for this observation rests on a failure to reject the null hypothesis, and relatively little of this research has employed the acoustic startle blink in human beings. The purpose of the present study was to provide additional evidence on this issue. Participants ( n = 20) encountered trials in which a prepulse (p) and two 103 dB(A) blink-eliciting noise bursts (S1 and S2) were given in succession. The prepulse (a synchronous word and tone) occurred 150 ms prior to S1. The prepulse inhibited the startle blink to S1, and S1 depressed the blink elicited 1.5 s later by S2. However, regardless of whether p inhibited the blink to S1, S1 maintained the same capacity to depress the blink to S2. In contrast, a softer S1 (88 dB(A); S1 attenuated), which produced a blink nearly matching the size of the prepulse-inhibited blink, did not significantly depress the response to S2. Participants also judged the loudness of S1 and S2. The prepulse reduced the perceived intensity of S1, but much less so than caused by reducing the actual intensity of S1, and proportionally much less so than the prepulse reduced the blink to S1. These results provide further evidence for independent reflex-eliciting and reflex-modifying effects of a startle-eliciting stimulus and argue against the notion that prepulses strongly reduce the general sensory impact of the startle stimulus.