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      Óxido nítrico na germinação de sementes de baixo vigor de Dalbergia nigra Translated title: Nitric oxide on the germination of Dalbergia nigra seeds with low vigor

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          Abstract

          O óxido nítrico é um composto que participa de inúmeros processos fisiológicos vegetais, dentre os quais atua estimulando a germinação tanto sob condições normais quanto sob estresse. O presente trabalho teve como objetivo investigar a eficiência de nitrato de potássio (KNO3) e de nitroprussiato de sódio (SNP), formadores de óxido nítrico, na germinação de sementes de baixo vigor de Dalbergia nigra, de modo a determinar as melhores condições de condicionamento das sementes da espécie. As sementes foram pré-embebidas em água ou nas soluções de KNO3 ou SNP nas seguintes concentrações: 0,01; 0,1; 1 e 10 mmol L-1, por períodos de tempo de seis, 12, 24 e 48-h. Em seguida, foram conduzidos testes de germinação em câmaras de germinação a 25 °C durante 12 dias, estimando-se a porcentagem de germinação pela contagem diária das sementes que emitiram radícula e o índice de velocidade de germinação (IVG). Nas sementes pré-embebidas em soluções de SNP e KNO3, as percentagens de germinação nas concentrações de 0,01 e 0,1 mmol L-1foram significativamente superiores às outras concentrações e à testemunha, que apresentou germinação média de 48%. Conclui-se que a aplicação de KNO3 e SNP aumentou a germinação e o vigor das sementes de Dalbergia nigra de baixo vigor.

          Translated abstract

          Nitric oxide is a compound that participates in many plant physiological processes, among which works by stimulating the germination both under normal and under stress conditions. This study aimed to investigate the effectiveness of potassium nitrate (KNO3) and sodium nitroprusside (SNP), forming nitric oxide, on the germination of seeds of low vigor of Dalbergia nigra, to determine the best conditions for the conditioning of the seeds of the species in these solutions. The seeds were pre-soaked in water or in solutions of KNO3 or SNP at the following concentrations: 0.01, 0.1, 1 and 10 mmol L-1, for periods of six, 12, 24 and 48 hours. Then, tests were conducted in germination chamber at 25 °C for 12 days, calculating the percentage of germination of seeds by counting daily that issued radicle and germination speed index (GSI). In pre-soaked seeds in solutions of KNO3 and SNP, the seed germination at concentrations of 0.1 and 1 mmol L-1was higher than the others, and also higher than the control, that had averaged 48% seed. It is concluded that the application of KNO3 and SNP increased seed germination and seed vigor of Dalbergia nigra seeds.

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          Most cited references32

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          Fisiologia de sementes de plantas cultivadas

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            Árvores Brasileiras-Manual de Identificação e Cultivo de Plantas Abóreas Nativas do Brasil

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              The Arabidopsis aleurone layer responds to nitric oxide, gibberellin, and abscisic acid and is sufficient and necessary for seed dormancy.

              Seed dormancy is a common phase of the plant life cycle, and several parts of the seed can contribute to dormancy. Whole seeds, seeds lacking the testa, embryos, and isolated aleurone layers of Arabidopsis (Arabidopsis thaliana) were used in experiments designed to identify components of the Arabidopsis seed that contribute to seed dormancy and to learn more about how dormancy and germination are regulated in this species. The aleurone layer was found to be the primary determinant of seed dormancy. Embryos from dormant seeds, however, had a lesser growth potential than those from nondormant seeds. Arabidopsis aleurone cells were examined by light and electron microscopy, and cell ultrastructure was similar to that of cereal aleurone cells. Arabidopsis aleurone cells responded to nitric oxide (NO), gibberellin (GA), and abscisic acid, with NO being upstream of GA in a signaling pathway that leads to vacuolation of protein storage vacuoles and abscisic acid inhibiting vacuolation. Molecular changes that occurred in embryos and aleurone layers prior to germination were measured, and these data show that both the aleurone layer and the embryo expressed the NO-associated gene AtNOS1, but only the embryo expressed genes for the GA biosynthetic enzyme GA3 oxidase.
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                Author and article information

                Contributors
                Role: ND
                Role: ND
                Role: ND
                Role: ND
                Journal
                rca
                Revista de Ciências Agrárias
                Rev. de Ciências Agrárias
                Sociedade de Ciências Agrárias de Portugal (Lisboa )
                0871-018X
                September 2015
                : 38
                : 3
                : 438-444
                Affiliations
                [1 ] Universidade Federal Rural do Rio de Janeiro Brasil
                [2 ] Universidade Federal de Viçosa Brasil
                [3 ] Universidade Federal de Santa Catarina Brasil
                [4 ] Universidade Federal de São João Del-Rei Brasil
                Article
                S0871-018X2015000300019
                1d42b106-eeca-4df0-8e25-dc3394768ba5

                http://creativecommons.org/licenses/by/4.0/

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                SciELO Portugal

                Self URI (journal page): http://www.scielo.mec.pt/scielo.php?script=sci_serial&pid=0871-018X&lng=en
                Categories
                AGRICULTURE, MULTIDISCIPLINARY

                General agriculture
                forest seeds,jacaranda-da-bahia,potassium nitrate,sodium nitroprusside,jacarandá-da-Bahia,nitrato de potássio,nitroprussiato de sódio, sementes florestais

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