Polyadenylate Polymerase Activity in Stationary and Growing Cell Cultures

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Abstract

Soluble polyadenylic acid (poly(A)) polymerase content of stationary and growing cell populations from a variety (6) of cell lines was determined. Cell populations from stationary cultures presented poly(A) polymerase values with a mean of 31 ± 12 enzyme units/mg protein. The mean value for growing cell populations were 62 ± 18 enzyme units per mg protein. A statistically significant difference was found between stationary and growing cell populations from the variety of cell lines examined (p < 0.1). The observed differences in poly(A) polymerase levels persisted after fractionation of the crude extracts and revealed two molecular forms of enzyme activity with a net charge difference in stationary and growing cell cultures.

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Journal

Journal ID (publisher-id): PAT

Journal ID (nlm-ta): Pathobiology

Journal ID (doi): 10.1159/issn.1015-2008

Title: Pathobiology

Publisher: S. Karger AG

ISSN (Print): 1015-2008

ISSN (Electronic): 1423-0291

Publication date Subscription-year: 1987

Publication date (Print): 1987

Publication date (Electronic): 07 October 2008

Volume: 55

Issue: 3

Pages: 164-172

Affiliations

^aDepartment of Biochemistry, Papanicolaou Research Center of Oncology, Athens; ^bDepartment of Biology, NRC-Democritos, Attiki, Greece

Article

Publisher ID: 163412 Other ID: Expl Cell Biol 1987;55:164–172

DOI: 10.1159/000163412

SO-VID: 1d475fa9-bf9c-41a1-ab6f-2e8d175d8a48

License:

Copyright: All rights reserved. No part of this publication may be translated into other languages, reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying, recording, microcopying, or by any information storage and retrieval system, without permission in writing from the publisher. Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in government regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug. Disclaimer: The statements, opinions and data contained in this publication are solely those of the individual authors and contributors and not of the publishers and the editor(s). The appearance of advertisements or/and product references in the publication is not a warranty, endorsement, or approval of the products or services advertised or of their effectiveness, quality or safety. The publisher and the editor(s) disclaim responsibility for any injury to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements.

History

Date received : 09 April 1987

Date accepted : 28 May 1987

Page count

Pages: 9

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