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      Microsatellites mapping for non-model species with chromosomal rearrangement: a case study in the frog Quasipaa boulengeri (Anura: Dicroglossidae).

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          Abstract

          Gene mapping is an important resource for understanding the evolution of genes and cytogenetics. Model species with a known genetic map or genome sequence allow for the selection of genetic markers on a desired chromosome, while it is hard to locate these markers on chromosomes of non-model species without such references. A frog species, Quasipaa boulengeri, shows chromosomal rearrangement polymorphisms, making itself a fascinating model for chromosomal speciation mediated by suppressed recombination. However, no markers have been located on its rearranged chromosomes. We present a complete protocol to map microsatellites based on mechanical microdissection and chromosome amplification techniques. Following this protocol, we mapped 71 microsatellites of Q. boulengeri at the chromosome level. In total, eight loci were assigned to rearranged chromosomes, and the other 63 loci might attach to other chromosomes. These microsatellites could be used to compare the gene flow and verify the chromosomal suppressed recombination hypothesis in Q. boulengeri. This integrated protocol could be effectively used to map genes to chromosomes for non-model species.

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          Author and article information

          Journal
          Genome
          Genome
          Canadian Science Publishing
          1480-3321
          0831-2796
          Aug 2017
          : 60
          : 8
          Affiliations
          [1 ] a Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu, China.
          [2 ] b University of Chinese Academy of Sciences, Beijing, China.
          Article
          10.1139/gen-2016-0200
          28727488
          1d4d7392-9ccd-4497-a051-4a2c2ee9ea15
          History

          chromosome rearrangement,recombination suppression,réarrangement chromosomique,suppression de la recombinaison,microsatellites mapping,Quasipaa boulengeri,Quaspiaa boulengeri,cartographie des microsatellites

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