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      Avaliação do potencial de uso da restrição hídrica em teste de sanidade de sementes de trigo visando à detecção de fungos Translated title: Potential of water restriction technique for wheat seed health testing

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          Abstract

          Objetivou-se, com o presente trabalho, avaliar a viabilidade do uso da restrição hídrica, em substituição ou alternativa ao uso da técnica de congelamento de sementes de trigo, no teste de incubação em substrato de papel, blotter test. O trabalho consistiu na comparação das duas técnicas por meio do exame de 60 amostras de sementes, coletadas em diferentes áreas do Estado de Minas Gerais e outras regiões do país, tendo como restritor hídrico o soluto manitol, no potencial de -1,10 MPa, valor este determinado preliminarmente com base na capacidade de redução da germinação das sementes de trigo em condições do teste de sanidade. A incidência média de Bipolaris sorokiniana não foi afetada por nenhuma das técnicas comparadas, havendo, por outro lado, um maior estímulo da restrição hídrica em relação à Pyricularia grisea, Aspergillus ochraceus, Cladosporium cladosporioides e Epicoccum purpuracens. Para Alternaria alternata, Drechslera tritici-repentis e Fusarium graminearum, os maiores valores de incidência ocorreram no método do congelamento.

          Translated abstract

          The objective of this study was to evaluate the use of the water restriction technique as an alternative to deep freezing for inhibiting wheat seed germination during blotter test incubation. The two techniques were compared using sixty seed samples collected in different areas of Minas Gerais state and other regions of Brazil. The water restriction established was determined by using manitol, at a potential of -1.10 MPa, based on the results of preliminary essays. The mean incidence of B. sorokiniana was not affected by any of the two techniques. Values for the incidence of Pyricularia grisea, Aspergillus ochraceus, Cladosporium cladosporioides and Epicoccum purpuracens were higher when water restriction was used. On the other hand, values for the incidence of Alternaria alternata, Drechslera tritici-repentis and Fusarium graminearum were lower with water restriction. Elongation of the seed radicle can be tolerated to a certain degree during blotter test incubation and the water restriction technique is a potential alternative of the freezing technique for detecting most of the fungi associated with wheat seeds.

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          Most cited references 13

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          Seed Pathology

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            Patologia de sementes: fundamentos e aplicações

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              Occurrence and Virulence of Bipolaris hawaiiensis on Bermudagrass (Cynodon dactylon) on Poultry Waste Application Sites in Mississippi

               R. G. Pratt (2001)
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                Author and article information

                Contributors
                Role: ND
                Role: ND
                Role: ND
                Role: ND
                Journal
                rbs
                Revista Brasileira de Sementes
                Rev. bras. sementes
                Associação Brasileira de Tecnologia de Sementes (Londrina )
                0101-3122
                2012
                : 34
                : 4
                : 613-618
                Affiliations
                [1 ] Celano Rep. Com. Ltda Brasil
                [2 ] Universidade Federal de Lavras Brazil
                [3 ] Universidade Estadual de Ponta Grossa Brazil
                [4 ] Universidade Federal de Lavras Brazil
                Article
                S0101-31222012000400012
                10.1590/S0101-31222012000400012

                http://creativecommons.org/licenses/by/4.0/

                Product
                Product Information: SciELO Brazil
                Categories
                PLANT SCIENCES

                Plant science & Botany

                blotter test, seed pathology, Triticum aestivum, patologia de sementes

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