Glucuronide metabolites of <i>trans</i>-ε-viniferin decrease triglycerides accumulation in an in vitro model of hepatic steatosis

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Abstract

Trans-ε-viniferin, a resveratrol dimer found mainly in grapevine wood, has shown protective capacities against hepatic steatosis in vivo. Nevertheless, this compound is very poorly bioavailable. Thus, the aim of the present study is to determine the potential anti-steatotic properties of 1 and 10 µM of trans-ε-viniferin and its four glucuronide metabolites in AML-12 cells treated with palmitic acid as an in vitro model of hepatic steatosis. The effect of the molecules in cell viability and triglyceride accumulation, and the underlying mechanisms of action by Real-Time PCR and Western Blot were analysed, as well as the quantification of trans-ε-viniferin and the identified bioactive metabolite inside cells and their incubation media. Interestingly, we were able to determine the triglyceride-lowering property of one of the glucuronides ( trans-ε-viniferin-2-glucuronide), which acts on de novo lipogenesis, fatty acid uptake and triglyceride assembly. The glucuronides of trans-ε-viniferin would therefore be partly responsible for the in vivo observed anti-steatotic properties of the parent compound.

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Author and article information

Contributors

Itziar Eseberri:

ORCID: http://orcid.org/0000-0001-5036-963X

itziar.eseberri@ehu.eus

Journal

Journal ID (nlm-ta): J Physiol Biochem

Journal ID (iso-abbrev): J Physiol Biochem

Title: Journal of Physiology and Biochemistry

Publisher: Springer Netherlands (Dordrecht )

ISSN (Print): 1138-7548

ISSN (Electronic): 1877-8755

Publication date (Electronic): 31 July 2024

Publication date PMC-release: 31 July 2024

Publication date (Print): 2024

Volume: 80

Issue: 3

Pages: 685-696

Affiliations

[1 ]UMR 1366, Univ. Bordeaux, Bordeaux INP, INRAE, ISVV, 33140 Villenave d’Ornon, OENO France

[2 ]GRID grid.434203.2, ISNI 0000 0001 0659 4135, UMR 1366, , Bordeaux Sciences Agro, Bordeaux INP, INRAE, ISVV, ; 33170 Gradignan, OENO France

[3 ]INSERM U1312, Bordeaux Institute of Oncology - BRIC, BioGo Team, Bordeaux, France

[4 ]Service de Biologie Des Tumeurs Et Tumorothèque, CHU de Bordeaux, ( https://ror.org/01hq89f96) Bordeaux, France

[5 ]Centre Antipoison Et de Toxicovigilance de Nouvelle Aquitaine, Bâtiment UNDR, CHU de Bordeaux, ( https://ror.org/01hq89f96) 33076 Bordeaux, France

[6 ]Nutrition and Obesity Group, Department of Pharmacy and Food Sciences, Faculty of Pharmacy, University of Basque Country (UPV/EHU) and Lucio Lascaray Research Centre, 01006 Vitoria-Gasteiz, Spain

[7 ]Bioaraba Health Research Institute, 01009 Vitoria-Gasteiz, Spain

[8 ]CIBEROBN Physiopathology of Obesity and Nutrition, Institute of Health Carlos III (ISCIII), ( https://ror.org/00ca2c886) 28029 Madrid, Spain

Author information

Samuel Amintas http://orcid.org/0000-0003-3238-5251

Stéphanie Krisa http://orcid.org/0000-0001-6509-8772

Arnaud Courtois http://orcid.org/0000-0002-0996-7133

Tristan Richard http://orcid.org/0000-0002-5308-8697

Itziar Eseberri http://orcid.org/0000-0001-5036-963X

Maria P. Portillo http://orcid.org/0000-0003-2290-592X

Article

Publisher ID: 1035

DOI: 10.1007/s13105-024-01035-w

PMC ID: 11502592

PubMed ID: 39085719

SO-VID: 1dae1087-5086-4317-a9cb-a00897933896

License:

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

History

Date received : 5 November 2023

Date accepted : 10 July 2024

Funding

Funded by: FundRef http://dx.doi.org/10.13039/501100003451, Euskal Herriko Unibertsitatea;

Award ID: IT1482-22

Funded by: FundRef http://dx.doi.org/10.13039/501100004587, Instituto de Salud Carlos III;

Award ID: CB12/03/30007

Funded by: FundRef http://dx.doi.org/10.13039/501100006251, Université de Bordeaux;

Award ID: the "Consortium Trans-Pyrénéen de Recherches sur l'Obésité et le Diabète"

Award ID: the "Nutrition Méditerranéenne et Santé"

Award ID: "Initiative d’Excellence"

Funded by: Universidad del País Vasco

Open Access :

Open Access funding provided thanks to the CRUE-CSIC agreement with Springer Nature.

Custom metadata

Keywords: trans-ε-viniferin,resveratrol dimer,glucuronide metabolites,aml-12-hepatocytes,liver steatosis

Data availability:

Keywords: trans-ε-viniferin, resveratrol dimer, glucuronide metabolites, aml-12-hepatocytes, liver steatosis

Glucuronide metabolites of trans-ε-viniferin decrease triglycerides accumulation in an in vitro model of hepatic steatosis

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