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      Anti-Idiotype Antibody Directly Interferes with Glomerular IgA Immune Complex Deposition

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          Data from both animal and clinical studies suggest that anti-idiotype antibodies deposited in glomeruli may be involved in the pathogenesis of glomerulonephritis. This study was conducted to examine the role of a hybridoma-AB1-2-derived IgG anti-T15 idiotype (IgG anti-T15) in the immunopathogenesis of a short-term experimental IgA nephropathy. BALB/c mice (12/group) were administered intravenously with: (1) an equal mass (1 mg) of T15-hybridoma-derived IgA antiphosphorylcholine (PC) and PC-conjugated bovine serum albumin (BSA-PC) antigen; (2) 1 mg of IgA anti-PC, 1 mg of BSA-PC antigen, and 3 mg of IgG anti-T15, or (3) 1 mg of BSA-PC antigen alone. The mice were sacrificed 6 h after the injection. A 6-hour clearance study was performed. The initial phase of elimination of BSA-PC antigen in mice receiving IgA anti-PC/BSA-PC/IgG anti-T15 or those receiving the antigen alone was significantly faster than that in those receiving IgA anti-PC/BSA-PC (p < 0.001). There was no significant difference in the elimination rate of BSA-PC antigen between mice receiving IgA anti-PC/BSA-PC/IgG anti-T15 and those receiving BSA-PC antigen alone. The late phases of elimination of the BSA-PC antigen in mice receiving IgA anti-PC/BSA-PC/IgG anti-T15 showed somewhat similar to those of BSA-PC antigen in mice receiving IgA anti-PC/BSA-PC. Moreover, mice injected with IgA anti-PC/BSA-PC/IgG anti-T15 showed a significantly less glomerular BSA-PC antigen deposition than those injected with IgA anti-PC/BSA-PC (positive control), as demonstrated by light microscopy, autoradiography, and immunohistochemistry (each p < 0.001). It is inferred that the injected IgG anti-T15 could react with the IgA anti-PC in vivo, directly interfering with immune complex formation by the IgA anti-PC and BSA-PC antigen, thereby resulting in diminished glomerular deposition of the BSA-PC antigen. These findings suggest that an anti-idiotype antibody may be protective in the immunopathogenesis of IgA nephropathy, because of its inhibitory effect on glomerular trapping of an antigen.

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          Most cited references 4

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          Preparation of phosphorylcholine-conjugated antigens

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            Chicken anti-protein A prevents Staphylococcus aureus protein A from binding to human and rabbit IgG in immunoassays and eliminates most false positive results

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              Serologic and molecular characterization of the T15 idiotype—I. Topologic mapping of idiotopes on tepc15


                Author and article information

                S. Karger AG
                April 1998
                26 March 1998
                : 78
                : 4
                : 464-473
                a Division of Experimental Pathology, Department of Pathology, Tri-Service General Hospital, b Department of Parasitology and Tropical Medicine, c Department of Medical Research, National Defense Medical Center, Taipei, Taiwan/ROC
                44976 Nephron 1998;78:464–473
                © 1998 S. Karger AG, Basel

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                Page count
                Figures: 6, Tables: 1, References: 50, Pages: 10
                Self URI (application/pdf):
                Original Paper

                Cardiovascular Medicine, Nephrology

                IgA nephropathy, Anti-idiotype antibodies, Idiotype


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