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      Hydrogel properties influence ECM production by chondrocytes photoencapsulated in poly(ethylene glycol) hydrogels

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      Journal of Biomedical Materials Research
      Wiley-Blackwell

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          Development of biocompatible synthetic extracellular matrices for tissue engineering.

          Tissue engineering may provide an alternative to organ and tissue transplantation, both of which suffer from a limitation of supply. Cell transplantation using biodegradable synthetic extracellular matrices offers the possibility of creating completely natural new tissues and so replacing lost or malfunctioning organs or tissues. Synthetic extracellular matrices fabricated from biocompatible, biodegradable polymers play an important role in the formation of functional new tissue from transplanted cells. They provide a temporary scaffolding to guide new tissue growth and organization, and may provide specific signals intended to retain tissue-specific gene expression.
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            Fluorometric assay of DNA in cartilage explants using Hoechst 33258.

            A simple two-step fluorometric assay of DNA in cartilage explants, utilizing the bisbenzimidazole dye Hoechst 33258, is described. Cartilage explants were prepared for assay by digestion with papain. Aliquots of the digest were mixed with dye solution, and the fluorescence emission measured. The enhancement in fluorescence of dye was specific for DNA, as demonstrated by 97% sensitivity to DNase and resistance to RNase. In addition, little or no interference was caused by non-DNA tissue components, since DNA caused an equal enhancement in fluorescence independent of the presence of papain-digested cartilage. By performing the assay on isolated chondrocytes, the cellular content of DNA was computed to be 7.7 pg per chondrocyte. The assay was stable for at least 2 h and sensitive to as little as 6 ng of DNA or equivalently less than 1000 cells. This procedure offers advantages over other established DNA assays of cartilage and may be especially useful in metabolic studies of cartilage explants.
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              Bioerodible hydrogels based on photopolymerized poly(ethylene glycol)-co-poly(.alpha.-hydroxy acid) diacrylate macromers

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                Author and article information

                Journal
                Journal of Biomedical Materials Research
                J. Biomed. Mater. Res.
                Wiley-Blackwell
                0021-9304
                1097-4636
                January 2002
                January 2002
                : 59
                : 1
                : 63-72
                Article
                10.1002/jbm.1217
                11745538
                1de0c95a-2f4d-447b-96ac-d8bb875f0580
                © 2002

                http://doi.wiley.com/10.1002/tdm_license_1.1

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