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      GH Values after Clonidine Stimulation Measured by Immunofluorometric Assay in Normal Prepubertal Children and GH-Deficient Patients

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          Objective: To establish the cut-off values of GH measured by immunofluorometric assay, a more sensitive and specific assay, in normal prepubertal children and compare their values with those of proven GH-deficient patients. Methods: 30 normal children (20 males) and 26 patients with known causes of GH deficiency were submitted to the clonidine test and their GH values were compared. A powdered clonidine tablet (0.1 mg/m<sup>2</sup>) was given orally and blood samples for GH measurements were drawn at times –30, 0, 60, 90 and 120 min. Results: GH peak values presented a wide variation ranging from 1.7 to 25 µg/l (mean ± SD = 12.87 ± 5.8 µg/l) in the normal group. The cut-off values for the 5th and 10th percentiles of the distribution curve were 3.3 and 5.5 µg/l, respectively. In the GH deficiency group, maximum GH levels after clonidine stimulation ranged from <0.1 to 2.1 µg/l (0.56 ± 0.58 µg/l). Conclusions: The cut-off values obtained with the immunofluorometric method are lower than the ones obtained by radioimmunoassay. We suggest a cut-off value of 3.3 µg/l (5th percentile) that ensures 100% of sensitivity along with 93% of specificity to exclude the diagnosis of GH deficiency when using this immunofluorometric method.

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          Most cited references 4

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          Consensus Guidelines for the Diagnosis and Treatment of Growth Hormone (GH) Deficiency in Childhood and Adolescence: Summary Statement of the GH Research Society

           G. Society (2000)
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            Diagnostic controversy: the diagnosis of childhood growth hormone deficiency revisited

             R Rosenfeld (1995)
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              Reliability of provocative tests to assess growth hormone secretory status. Study in 472 normally growing children

               E. Ghigo (1996)

                Author and article information

                Horm Res Paediatr
                Hormone Research in Paediatrics
                S. Karger AG
                08 May 2003
                : 59
                : 5
                : 229-233
                Unidade de Endocrinologia do Desenvolvimento e Laboratório de Hormônios e Genética Molecular LIM /42, Hospital das Clínicas da Faculdade de Medicina da Universidade de São Paulo, Brazil
                70222 Horm Res 2003;59:229–233
                © 2003 S. Karger AG, Basel

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                Page count
                Tables: 4, References: 31, Pages: 5
                Original Paper


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