Jong-Chan Youn , 1 , 2 , Jun Yong Kim 2 , Min Kyung Jung 2 , Hee Tae Yu 2 , 3 , Su-Hyung Park 4 , In-Cheol Kim 5 , Sun Ki Lee 1 , Suk-Won Choi 1 , Seongwoo Han 1 , Kyu-Hyung Ryu 1 , Sungha Park 3 , Eui-Cheol Shin , 2
20 March 2018
Recent studies suggest an association between cytomegalovirus (CMV) infection and hypertension. In the present study, we used a variety of antigens and different assay methods to investigate the relationship between CMV-specific T-cell responses and arterial stiffness in patients with hypertension.
To evaluate arterial stiffness, pulse wave velocity (PWV) was measured in 207 hypertensive patients (average age, 63 ± 8 years). To measure CMV pp65 and IE-1-specific T-cell responses, we performed intracellular cytokine staining (ICS) and enzyme-linked immunospot (ELISPOT) assays. We also analyzed CMV-specific T-cell responses against 10 different CMV antigens using ELISPOT assays.
In patients with hypertension, senescent CD8 + T-cell frequencies were significantly correlated with arterial stiffness. Moreover, arterial stiffness was independently associated with CMV pp65-specific CD8 + T-cell responses as measured by ICS. CMV-specific CD8 + T-cell responses measured by ICS and ELISPOT assays showed good agreement and significant correlation with each other. ELISPOT analyses against 10 different CMV antigens revealed a consistent response pattern irrespective of age, gender, and diabetes
CMV pp65-specific CD8 + T-cell responses were independently correlated with arterial stiffness in patients with hypertension. Additionally, the results of ICS and ELISPOT assays showed a significant correlation and good agreement with each other. These findings are important for guiding choices regarding the broad clinical application of CMV-specific T-cell response assays in this patient population.