Lipidomics unveils the complexity of the lipidome in metabolic diseases

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Abstract

Dysregulation of lipid metabolism is responsible for pathologies of human diseases including metabolic diseases. Recent advances in lipidomics analysis allow for the targeted and untargeted identification of lipid species and for their quantification in normal and diseased conditions. Herein, this review provides a brief introduction to lipidomics, highlights its application to characterize the lipidome at the cellular and physiological levels under different biological conditions, and discusses the potential for the use of lipidomics in the discovery of biomarkers.

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Most cited references 71

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MS-DIAL: Data Independent MS/MS Deconvolution for Comprehensive Metabolome Analysis

Hiroshi Tsugawa, Tomas Cajka, Tobias Kind … (2015)

Data-independent acquisition (DIA) in liquid chromatography tandem mass spectrometry (LC-MS/MS) provides more comprehensive untargeted acquisition of molecular data. Here we provide an open-source software pipeline, MS-DIAL, to demonstrate how DIA improves simultaneous identification and quantification of small molecules by mass spectral deconvolution. For reversed phase LC-MS/MS, our program with an enriched LipidBlast library identified total 1,023 lipid compounds from nine algal strains to highlight their chemotaxonomic relationships.

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The lipid mediator protectin D1 inhibits influenza virus replication and improves severe influenza.

Masayuki Morita, Keiji Kuba, Akihiko Ichikawa … (2013)

Influenza A viruses are a major cause of mortality. Given the potential for future lethal pandemics, effective drugs are needed for the treatment of severe influenza such as that caused by H5N1 viruses. Using mediator lipidomics and bioactive lipid screen, we report that the omega-3 polyunsaturated fatty acid (PUFA)-derived lipid mediator protectin D1 (PD1) markedly attenuated influenza virus replication via RNA export machinery. Production of PD1 was suppressed during severe influenza and PD1 levels inversely correlated with the pathogenicity of H5N1 viruses. Suppression of PD1 was genetically mapped to 12/15-lipoxygenase activity. Importantly, PD1 treatment improved the survival and pathology of severe influenza in mice, even under conditions where known antiviral drugs fail to protect from death. These results identify the endogenous lipid mediator PD1 as an innate suppressor of influenza virus replication that protects against lethal influenza virus infection. Copyright © 2013 Elsevier Inc. All rights reserved.

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Global analyses of cellular lipidomes directly from crude extracts of biological samples by ESI mass spectrometry: a bridge to lipidomics.

Xianlin Han, Kenneth W. Gross (2003)

Lipidomics is a rapidly expanding research field in which multiple techniques are utilized to quantitate the hundreds of chemically distinct lipids in cells and determine the molecular mechanisms through which they facilitate cellular function. Recent developments in electrospray ionization mass spectrometry (ESI/MS) have made possible, for the first time, the precise identification and quantification of alterations in a cell's lipidome after cellular perturbations. This review provides an overview of the essential role of ESI/MS in lipidomics, presents a broad strategy applicable for the generation of lipidomes directly from cellular extracts of biological samples by ESI/MS, and summarizes salient examples of strategies utilized to conquer the lipidome in physiologic signaling as well as pathophysiologically relevant disease states. Because of its unparalleled sensitivity, specificity, and efficiency, ESI/MS has provided a critical bridge to generate highly accurate data that fingerprint cellular lipidomes to facilitate insight into the functional role of subcellular membrane compartments and microdomains in mammalian cells. We believe that ESI/MS-facilitated lipidomics has now opened a critical door that will greatly increase our understanding of human disease.

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Author and article information

Contributors

Todd A. Lydic: 518-353-1119 , lydictod@msu.edu

Young-Hwa Goo:

ORCID: http://orcid.org/0000-0003-1680-1569

518-262-1159 , gooy@amc.edu

Journal

Journal ID (nlm-ta): Clin Transl Med

Journal ID (iso-abbrev): Clin Transl Med

Title: Clinical and Translational Medicine

Publisher: Springer Berlin Heidelberg (Berlin/Heidelberg )

ISSN (Electronic): 2001-1326

Publication date (Electronic): 26 January 2018

Publication date PMC-release: 26 January 2018

Publication date Collection: 2018

Volume: 7

Electronic Location Identifier: 4

Affiliations

[1 ]ISNI 0000 0001 0427 8745, GRID grid.413558.e, Department of Molecular and Cellular Physiology, , Albany Medical College, ; 47 New Scotland Avenue, Albany, NY 12208 USA

[2 ]ISNI 0000 0001 2150 1785, GRID grid.17088.36, Department of Physiology, , Michigan State University, ; East Lansing, MI 48824 USA

Author information

Young-Hwa Goo http://orcid.org/0000-0003-1680-1569

Article

Publisher ID: 182

DOI: 10.1186/s40169-018-0182-9

PMC ID: 5786598

PubMed ID: 29374337

SO-VID: 1efa88e6-2fa2-4f19-8833-69858f56c46f

License:

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License ( http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

History

Date received : 6 November 2017

Date accepted : 8 January 2018

Funding

Funded by: FundRef http://dx.doi.org/10.13039/100000968, American Heart Association;

Award ID: 14SDG19690016

Award Recipient : Young-Hwa Goo

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ScienceOpen disciplines: Medicine

Keywords: lipid,lipidomics,lipidome,metabolism,metabolic disease,lipoprotein,lipid droplet,atherosclerosis,mass spectrometry

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ScienceOpen disciplines: Medicine

Keywords: lipid, lipidomics, lipidome, metabolism, metabolic disease, lipoprotein, lipid droplet, atherosclerosis, mass spectrometry

Lipidomics unveils the complexity of the lipidome in metabolic diseases

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Point-of-Care Testing for Infectious Diseases Super Collection

Most cited references 71

MS-DIAL: Data Independent MS/MS Deconvolution for Comprehensive Metabolome Analysis

The lipid mediator protectin D1 inhibits influenza virus replication and improves severe influenza.

Global analyses of cellular lipidomes directly from crude extracts of biological samples by ESI mass spectrometry: a bridge to lipidomics.

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