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      Re-record and Spore Ultrastructure of Nosema melasomae Sidor & Jodal 1986, a Microsporidian Pathogen of Crysomela populi (Coleoptera: Chrysomelidae)


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          Chrysomela (=Melasoma) populi is one of the most serious pests on poplar plantations. In the present study, a microsporidian pathogen, Nosema melasomae infecting Crysomela populi is re-recorded from a new geographical locality and its spore ultrastructure is given for the first time.


          Larvae and adults of C. populi were dissected in Ringer’s solution and prepared wet smears were examined under a microscope. Detected fresh and stained spores were measured and photographed using an Olympus BX51 microscope with a DP-25 digital camera and a DP2-BSW Soft Imaging System. The ultrastructure of the pathogen was studied with a Philips EM 208 transmission electron microscope using standard preparation techniques as previously described


          Fresh spores of the microsporidian pathogen are elongate, 4.86 ± 0.71 μm in length and 1.64 ± 0.19 μm in width. The spore wall is considerable thin, measured 60 to 100 nm and consists of a clear endospore (40 to 80 nm) and an electron-dense, uniform exospore (15 to 30 nm). The polar filament is isofilar and has only 6–8 coils. Nuclei in the cell are 400–560 nm in diameter. The polaroplast has a thin lamellated structure.


          The pathogen from C. populi is N. melasomae Sidor & Jodal, 1986 and its systematic position given by Sidor and Jodal. The spore ultrastructure of N. melasomae differs from those of other microsporidia infecting chrysomelids.

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          Most cited references24

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          Die Microsporidien als Parasiten der Insekten.

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            Nosema galerucellae n. sp., Microsporidian (Protozoa, Microspora), parasite of Galerucella luteola Müller (Chrysomelidae, Coleoptera): Development cycle and ultrastructure

            The life cycle of the microsporidian parasite of Galerucella luteola (Chrysomelidae, Coleoptera) was investigated by optical and electron microscopy. All stages were diplokaryotic and apansporoblastic. Sporogony was disporous. Spores were oval, elongated and measured 4.95 × 2.89 μm with a lamellar polaroplast and 7-9 coils of polar filament. These ultrastructural features indicate that this microsporidian is a Nosema. We have named it Nosema galerucellae n. sp.
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              A new isolate of Nosema sp. (Microsporidia, Nosematidae) from Phyllobrotica armata Baly (Coleoptera, Chrysomelidae) from China

              We studied the spore morphology and molecular systematics of a novel microsporidian isolate from Phyllobrotica armata Baly collected in China. The spores were long-oval and measured 4.7 × 2.6 μm on fresh smears. Ultrastructure of the spores was characteristic for the genus Nosema: 13-14 polar filament coils, posterior vacuole, and a diplokaryon. The complete rRNA gene sequence of the isolate was 4308 bp long. The organization of the rRNA gene was 5'-LSU rRNA-ITS-SSU rRNA-IGS-5S-3', which corresponds to that of the Nosema species. Phylogenetic analysis based on the rRNA gene sequence indicated that this isolate, designated as Nosema sp. PA, is closely related to Nosema bombycis and is correctly assigned to the "true"Nosema group.

                Author and article information

                Iran J Parasitol
                Iran J Parasitol
                Iranian Journal of Parasitology
                Tehran University of Medical Sciences
                Apr-Jun 2018
                : 13
                : 2
                : 244-250
                [1. ] Dept. of Biology, Faculty of Science, Karadeniz Technical University, 61080, Trabzon, Turkey
                [2. ] Faculty of Arts and Science, Abant İzzet Baysal University, 14030, Bolu, Turkey
                Author notes
                [* ] Correspondence Email: muyaman@ 123456hotmail.com
                Copyright© Iranian Society of Parasitology & Tehran University of Medical Sciences

                This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

                : 07 April 2017
                : 16 August 2017
                Original Article

                nosema melasomae,sporeultrastructure,microsporidium,crysomela populi,chrysomelidae
                nosema melasomae, sporeultrastructure, microsporidium, crysomela populi, chrysomelidae


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