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RhoH is required to maintain the integrin LFA-1 in a nonadhesive state on lymphocytes.

Nature immunology

genetics, immunology, Electrophoresis, Polyacrylamide Gel, Flow Cytometry, Humans, Intercellular Adhesion Molecule-1, metabolism, Jurkat Cells, Lymphocyte Function-Associated Antigen-1, Mutation, RNA, Messenger, analysis, Signal Transduction, T-Lymphocytes, Transcription Factors, rho GTP-Binding Proteins, Blotting, Southern, Cell Adhesion

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      Abstract

      Lymphocyte function-associated antigen 1 (LFA-1) is relatively nonadhesive on resting lymphocytes; however, the mechanisms underlying changes in its adhesiveness are poorly understood. In this study, we generated a Jurkat T cell clone, J+hi1.14, that contained low amounts of mRNA for RhoH, a leukocyte-specific inhibitory Rho family member. J+hi1.14 cells expressed constitutively adhesive LFA-1 and the cells bound spontaneously to intracellular adhesion molecules 1, 2 and 3. Reconstitution of RhoH mRNA expression in J+hi1.14 cells reverted the adhesion phenotype to that of wild-type. We obtained similar results using RNA interference in peripheral blood lymphocytes. These data demonstrate that RhoH is required for maintenance of lymphocyte LFA-1 in a nonadhesive state.

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      Most cited references 41

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        Point mutations define a sequence flanking the AUG initiator codon that modulates translation by eukaryotic ribosomes.

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          Adhesion receptors of the immune system.

           T. Springer (1990)
          The adhesive interactions of cells with other cells and with the extracellular matrix are crucial to all developmental processes, but have a central role in the functions of the immune system throughout life. Three families of cell-surface molecules regulate the migration of lymphocytes and the interactions of activated cells during immune responses.
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            Author and article information

            Journal
            10.1038/ni1103
            15300248

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