Ítalo Diego Rebouças de Araújo , 1 , Nayara Coriolano de Aquino 2 , Andreza Conceição Véras de Aguiar Guerra 3 , Renato Ferreira de Almeida Júnior 4 , Renata Mendonça Araújo 5 , Raimundo Fernandes de Araújo Júnior 3 , Kléber Juvenal Silva Farias 6 , José Veríssimo Fernandes 1 , Vânia Sousa Andrade 1
22 August 2017
This study evaluated the in vitro activity of essential oil extracted from the leaves of Myracrodruon urundeuva.
The oil was obtained by hydro-distillation and characterized by Gas Chromatography coupled to Mass Spectrometry (GC-MS) and Gas Chromatography with Flame Ionization Detector (GC-FID). The antibacterial activity was evaluated by the broth microdilution technique and the MIF was determined by using growth indicator CTT (2,3,5-triphenyl-tetrazolium) and CBM in BHI agar. The oil’s cytotoxicity was evaluated in HeLa, HEK-293, and Vero E6 cells using MTT, 3- (4,5-dimethylthiazol-2-yl) -2,5-diphenyl tetrazolium.
The oil showed chemical markers, including α-pinene (87.85%), trans-caryophyllene (1.57%), limonene (1.49%) and β -pinene (1.42%), and activity against all strains: Staphylococcus aureus (MIC = MBC = 0.22 mg/mL), Staphylococcus epidermidis (MIC = 0.11 mg/mL and MBC = 0.22 mg/mL), Escherichia coli (MIC = 0.88 mg/mL and MBC = 1.75 mg/mL), Pseudomonas aeruginosa (MIC = MBC = 7 mg/mL) and Salmonella Enteritidis (MIC = MBC = 0.44 mg/mL). In vitro cytotoxicity tests showed that the oil is not toxic and has slight antitumor activity.