Background: Acute and early diagnosed hepatitis C virus (HCV) infections are rare diagnoses. Patients on regular dialysis treatment (RDT) are at risk of acquiring HCV infection. Aims of the Study: (1) To determine the efficacy and safety of two-phase induction treatment of acute and early diagnosed HCV infections in patients on RDT, and (2) to establish the importance of serum HCV RNA testing at defined time points of treatment for the prediction of the therapeutic effect. Therapeutic Protocol: Antiviral treatment consisted of two different phases: phase A therapy was interferon (IFN)-α2b 10 million units (MU) s.c. administered daily for 21 days followed by phase B with IFN-α2b 3 MU s.c. administered 3 times weekly for 12 weeks. Results: (1) Efficacy of the treatment: A sustained virological response (SVR) was achieved in a total of 13/18 patients (72%). Safety: We did not observe any serious side effects of the treatment. The most pronounced side effect was the myelosuppression caused by IFN-α. (2) SVR prediction: Patients with negative serum HCV RNA at day 6 achieve SVR more frequently than those with positive HCV RNA at day 6 (p = 0.074). Conclusions: Treatment of acute and early diagnosed HCV infections in hemodialyzed patients is much more effective than treatment of chronic infection. Even relatively high doses of IFN at the beginning of therapy (10 MU daily) are tolerated well by the patients.