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      A comparison of transcriptomic profiles in endometrium during window of implantation between women with unexplained recurrent implantation failure and recurrent miscarriage.

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          Abstract

          The endometrium becomes receptive to the embryo only in the mid-luteal phase, but not in the other stages of the menstrual cycle. Endometrial factors play an important role in implantation. Women with recurrent miscarriage and recurrent implantation failure have both been reported to have altered expression of receptivity markers during the window of implantation. We aimed to compare the gene expression profiles of the endometrium in the window of implantation among women with unexplained recurrent implantation failures (RIF) and unexplained recurrent miscarriages (RM) by RNA sequencing (RNA-Seq). In total 20 patients (9 RIF and 11 RM) were recruited. In addition 4 fertile subjects were included as reference. Endometrium samples were precisely timed on the 7th day after luteal hormone surge (LH + 7). All the 24 endometrium samples were extracted for total RNA. The transcriptome was determined by RNA-Seq in the first 14 RNA samples (5 RIF, 6 RM and 3 fertile). Differentially expressed genes between RM and RIF were validated by quantitative real-time PCR (qPCR) in all 24 RNA samples (9 RIF, 11 RM and 4 fertile). Transcriptomic profiles of RM and RIF, but not control samples, were separated from each other by principle component analysis (PCA) and support vector machine (SVM). Complementary and coagulation cascades pathway was significantly up-regulated in RIF while down-regulated in RM. Differentially expressed genes C3, C4, C4BP, DAF, DF and SERPING1 in complement and coagulation cascade pathway between RM and RIF were further validated by qPCR. This study compared endometrial transcriptome among patients with RIF and RM in the window of implantation; it identified differential molecular pathways in endometrium between RIF and RM, which potentially affect the implantation process.

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          Author and article information

          Journal
          Reproduction
          Reproduction (Cambridge, England)
          BioScientifica
          1741-7899
          1470-1626
          Jun 2017
          : 153
          : 6
          Affiliations
          [1 ] Department of Obstetrics and Gynaecology.
          [2 ] School of Life SciencesThe Chinese University of Hong KongHong Kong SAR, China.
          [3 ] Reproductive Medicine Centre of the Affiliated HospitalGuilin Medical University, Guilin, Guangxi, China.
          [4 ] Biomolecular Sciences Research CentreSheffield Hallam University, Sheffield, UK.
          [5 ] Li Ka Shing Institute of Health Sciences.
          [6 ] School of Biomedical SciencesThe Chinese University of Hong Kong, Hong Kong SAR, China.
          [7 ] Department of Obstetrics and Gynaecology tinchiu.li@cuhk.edu.hk.
          Article
          REP-16-0574
          10.1530/REP-16-0574
          28283674
          1f72fd6b-cd87-4c46-9186-3700634c1531
          History

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