5
views
0
recommends
+1 Recommend
1 collections
    0
    shares
      • Record: found
      • Abstract: found
      • Article: found
      Is Open Access

      Variación estacional de las características seminales del bagre rayado Pseudoplatystoma metaense (Telostei, pimelodidae) Translated title: Stational variation on seminal characteristics in bagre rayado Pseudoplatystoma metaense (Telostei, pimelodidae)

      research-article

      Read this article at

      Bookmark
          There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.

          Abstract

          Objetivo. Determinar la variación de la calidad, el contenido iónico y la osmolaridad del plasma seminal de Pseudoplatystoma metaense durante la estación reproductiva. Materiales y métodos. Se utilizaron machos sexualmente maduros durante un periodo reproductivo (marzo a agosto). La espermiación fue inducida con Extracto de Hipófisis de Carpa (4 mg/kg). Se evaluó el volumen (mL), movilidad masal (%), tiempo de activación (sg), espermatocrito (%); concentración espermática (10(6) sptz/µL) y viabilidad (%). También fue determinada la movilidad (%) y velocidad individual por medio de un Sistema de Análisis Espermático Asistido por Computador (CASA). La osmolaridad (mOsm/Kg) y la concentración de iones (Na+, Cl+, K+ y Mg2+) y glucosa fue determinada en plasma seminal obtenido por centrifugación del semen a 14.000 g. La concentración de iones y glucosa fue establecida a través de un sistema de reflectancia. Resultados. El semen de Pseudoplatystoma metaense presentó una movilidad masal mayor al 90%, con el menor valor de movilidad progresiva lineal rápida para el mes de abril (34.9±9.0%) y en general valores de espermatozoides inmóviles menores al 10%. El plasma seminal mostró una osmolaridad de 259.3± 3.5 mOsm/Kg, con una concentración promedio de glucosa e iones Na+, Cl+, K+ y Mg2+ de 142.7±2.8, 118±2.2, 4.8±0.4 y 0.33±0.0 mmol/L, respectivamente, durante la época reproductiva. Conclusiones. La calidad seminal de bagre rayado no presentó variaciones en la estación reproductiva mostrando una alta calidad para los procesos de fecundación.

          Translated abstract

          Objective. To determine the quality variation, the ionic content and the osmolality in Pseudoplatystoma metaense seminal plasma during the reproductive period. Materials and methods. Sexually mature males were used during a reproductive season (march to august). The spermiation was induced with Carp Pituitary Extract (4 mg/kg). Volume (mL), masal motility (%), activation time (sc), spermatocrit (%), sperm concentration (10(6) sptz/µL) and viability (%) were evaluated. Also, motility (%) and individual speed through a Computer-Assisted Semen Analysis (CASA) were determined. The Osmolarity (mOsm/kg), the ions (Na+, Cl+, K+ and Mg2+) and the glucose concentration were determined in seminal plasma obtained by centrifugation at 14.000 g. The ions and glucose concentration were established through a reflectance system. Results. Sperm showed masal motility greater than 90%, with the lowest rapid linear progressive motility in the month of april (34.9±9.0%) and general levels of spermatozoa inmotile less than 10%. The seminal plasma showed an osmolarity of 259.3±3.5 mOsm/kg, with an average concentration of glucose and ions Na+, Cl+, K+ and Mg2+ of 142.7±2.8, 118±2.2, 4.8±0.4 and 0.33±0.0 mmol/L, respectively, during the reproductive season. Conclusions. The semen quality of bagre rayado presented no changes during the breeding season, and showed a high quality for the process of fertilization.

          Related collections

          Most cited references38

          • Record: found
          • Abstract: found
          • Article: not found

          Broodstock management and hormonal manipulations of fish reproduction.

          Control of reproductive function in captivity is essential for the sustainability of commercial aquaculture production, and in many fishes it can be achieved by manipulating photoperiod, water temperature or spawning substrate. The fish reproductive cycle is separated in the growth (gametogenesis) and maturation phase (oocyte maturation and spermiation), both controlled by the reproductive hormones of the brain, pituitary and gonad. Although the growth phase of reproductive development is concluded in captivity in most fishes-the major exemption being the freshwater eel (Anguilla spp.), oocyte maturation (OM) and ovulation in females, and spermiation in males may require exogenous hormonal therapies. In some fishes, these hormonal manipulations are used only as a management tool to enhance the efficiency of egg production and facilitate hatchery operations, but in others exogenous hormones are the only way to produce fertilized eggs reliably. Hormonal manipulations of reproductive function in cultured fishes have focused on the use of either exogenous luteinizing hormone (LH) preparations that act directly at the level of the gonad, or synthetic agonists of gonadotropin-releasing hormone (GnRHa) that act at the level of the pituitary to induce release of the endogenous LH stores, which, in turn act at the level of the gonad to induce steroidogenesis and the process of OM and spermiation. After hormonal induction of maturation, broodstock should spawn spontaneously in their rearing enclosures, however, the natural breeding behavior followed by spontaneous spawning may be lost in aquaculture conditions. Therefore, for many species it is also necessary to employ artificial gamete collection and fertilization. Finally, a common question in regards to hormonal therapies is their effect on gamete quality, compared to naturally maturing or spawning broodfish. The main factors that may have significant consequences on gamete quality-mainly on eggs-and should be considered when choosing a spawning induction procedure include (a) the developmental stage of the gonads at the time the hormonal therapy is applied, (b) the type of hormonal therapy, (c) the possible stress induced by the manipulation necessary for the hormone administration and (d) in the case of artificial insemination, the latency period between hormonal stimulation and stripping for in vitro fertilization. Copyright 2009 Elsevier Inc. All rights reserved.
            Bookmark
            • Record: found
            • Abstract: not found
            • Article: not found

            The measurement of sperm motility and factors affecting sperm quality in cultured fish

              Bookmark
              • Record: found
              • Abstract: not found
              • Article: not found

              Computer-assisted sperm analysis (CASA) as a tool for monitoring sperm quality in fish

                Bookmark

                Author and article information

                Journal
                mvz
                Revista MVZ Córdoba
                Rev.MVZ Cordoba
                Universidad de Córdoba - Facultad de Medicina Veterinaria y Zootecnia. (Córdoba, Montería, Colombia )
                0122-0268
                1909-0544
                January 2011
                : 16
                : 1
                : 2336-2348
                Affiliations
                [01] orgnameUniversidad de los Llanos orgdiv1Facultad de Ciencias Agropecuarias y Recursos Naturales orgdiv2Instituto de Acuicultura de la Universidad de los Llanos
                Article
                S0122-02682011000100009 S0122-0268(11)01600109
                1f7c2c73-4a8d-46fa-aa79-7de29d921f48

                This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

                History
                : November 2009
                : July 2010
                Page count
                Figures: 0, Tables: 0, Equations: 0, References: 30, Pages: 13
                Product

                SciELO Colombia

                Categories
                Originales

                reproduction,sperm,Pseudoplatystoma metaense,reprodución,semen

                Comments

                Comment on this article

                Similar content305

                Cited by5

                Most referenced authors261