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      The HVJ Liposome Method

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          Recent advancement of gene technology allows us a practical approach to gene therapy. Among various in vivo gene transfer techniques available, the HVJ liposome method is an efficient procedure which could target the glomerular cells. Using this method, HVJ-mediated cell fusion activity enables us to introduce genetic materials directly into the cytosol without degradation. In addition, cointroduction of non-histone nuclear protein, high-mobility group (HMG-1), efficiently facilitates migration of foreign DNA to the nucleus. Although there still exist some limitations, the HVJ liposome method may be applicable to the treatment of glomerular diseases as well as to analysis of the molecular aspects of renal pathophysiology.

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          Most cited references 3

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          Transforming growth factor beta in tissue fibrosis.

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            Cytoplasmic domains of the interleukin-2 receptor beta and gamma chains mediate the signal for T-cell proliferation.

            The interleukin-2 receptor (IL-2R) consists of three distinct chains (alpha, beta, gamma) which bind IL-2 and generate a proliferative signal in T cells. To define the mechanism of receptor activation, chimaeric receptors were constructed from the intracellular region of either IL-2R beta or IL-2R gamma and the extracellular region of c-kit, a receptor tyrosine kinase that homodimerizes on binding stem cell factor (SCF). We report here that binding of SCF to the beta-chain chimaera induced proliferation of the pro-B-cell line BA/F3, but not T cells. But in T cells expressing both the beta- and gamma-chain chimaeras, SCF induced proliferation and tyrosine phosphorylation characteristic of the native IL-2R signal. Chimaeric IL-2 receptor beta and gamma chains constructed with the heterodimeric extracellular regions of the granulocyte-macrophage colony stimulating factor receptor (GM-CSFR) also provided the IL-2R signal. Thus, heterodimerization of the cytoplasmic domains of IL-2R beta and -gamma appears necessary and sufficient for signalling in T cells.
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              In Vivo Transfection of Genes for Renin and Angiotensinogen into the Glomerular Cells Induced Phenotypic Change of the Mesangial Cells and Glomerular Sclerosis

               M. Arai,  A Wada,  Y Isaka (1995)

                Author and article information

                Nephron Exp Nephrol
                Cardiorenal Medicine
                S. Karger AG
                April 1998
                20 March 1998
                : 6
                : 2
                : 144-147
                a 1st Department of Medicine, Osaka University School of Medicine, Osaka, and b Institute for Molecular and Cellular Biology, Osaka University, Osaka, Japan
                20515 Exp Nephrol 1998;6:144–147
                © 1998 S. Karger AG, Basel

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                Page count
                Figures: 1, Tables: 1, References: 12, Pages: 4
                Self URI (application/pdf): https://www.karger.com/Article/Pdf/20515
                Technical Seminar: Tissue Targeting


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