Hironosuke Sakamoto a , Tetsuo Sakamaki b , Tsugiyasu Kanda a , Yo-ichi Hoshino c , Yoshie Sawada b , Mahito Sato a , Hiroko Sato c , Yuko Oyama c , Akihiko Nakano c , Shin-ichi Takase c , Akira Hasegawa c , Ryozo Nagai d , Masahiko Kurabayashi c
17 October 2003
Atherectomy specimens offer an opportunity to study the biology of coronary artery lesions. We cultured smooth muscle cells (SMCs) from specimens obtained from 24 patients with coronary restenosis after angioplasty to study the relationship between activity of SMCs (in vitro outgrowth) and the time course of restenosis. We also examined expression of a Kruppel-like zinc-finger transcription factor 5 (KLF; also known as BTEB2 and IKLF), which is markedly induced in activated SMCs, in the same specimens. SMC outgrowth was observed in 9 of 24 specimens (37.5%). Restenosis occurred sooner (p < 0.01) in patients whose specimens showed outgrowth compared to those whose specimens showed no outgrowth. Immunostaining for KLF5 was more common in specimens with outgrowth (89 vs. 20%, p < 0.01). These data suggest that the number of activated SMCs in lesions may determine in vitro outgrowth and also affect the time to restenosis.