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      Diversity of Microorganisms Isolated from the Soil Sample surround Chroogomphus rutilus in the Beijing Region

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          Abstract

          Artificially cultivating Chroogomphus rutilus is too inefficient to be commercially feasible. Furthermore, isolating C. rutilus mycelia in the wild is difficult. Thus, it is important to determine the natural habitat of its fruiting body. This study focused on the ecology of the C. rutilus habitat to isolate and classify beneficial microorganisms that could affect its growth, which could be used in future research on artificial cultivation. In total, 342 isolates were isolated from soil samples collected around a C. rutilus colony in the Beijing region. Of these, 22 bacterial and 14 fungal isolates were selected for sequencing and phylogenetic analysis, based on their growth characteristics and colony morphology. Using 16S rRNA gene sequence analysis, the bacterial isolates were divided into two monophyletic clusters which had significant hits to the genera Bacillus and Pseudomonas, respectively. Using internal transcribed spacer (ITS) sequence analysis, fungal isolates were divided into four monophyletic clusters: Penicillium, Trichoderma, Mortierella, and Bionectria. Moreover, the phylogenetic diversity of these isolates was analysed. The results indicated that numerous microorganisms were present in C. rutilus habitat. This was the first reported examination of the microbiological ecology of C. rutilus.

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              A rapid DNA isolation procedure for small quantities of fresh leaf tissues

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                Author and article information

                Journal
                Int J Biol Sci
                ijbs
                International Journal of Biological Sciences
                Ivyspring International Publisher (Sydney )
                1449-2288
                2011
                2 March 2011
                : 7
                : 2
                : 209-220
                Affiliations
                1. Institute of Plant and Environmental Protection, Beijing Academy of Agriculture and Forestry Science, Beijing 100097, China;
                2. College of Life Sciences, Henan Agricultural University, Zhengzhou 450002, Henan, China;
                3. College of Horticulture, Agricultural University of Hebei, Baoding 071001, Hebei, China;
                4. Department of Molecular Biology, University of Aarhus, Aarhus C DK-8000, Denmark
                Author notes
                ✉ Corresponding author: Feng Xu. Institute of Plant and Environmental Protection, Beijing Academy of Agriculture and Forestry Science, Beijing 100097, China. Tel: +86 10 51503432, Fax: +86 10 51503899, E-mail: xxu1022@ 123456163.com .

                Conflict of Interests: The authors have declared that no conflict of interest exists.

                Article
                ijbsv07p0209
                3053533
                21448282
                20b036f7-01d6-425c-8251-c8086b1376c7
                © Ivyspring International Publisher. This is an open-access article distributed under the terms of the Creative Commons License (http://creativecommons.org/licenses/by-nc-nd/3.0/). Reproduction is permitted for personal, noncommercial use, provided that the article is in whole, unmodified, and properly cited.
                History
                : 16 January 2011
                : 27 February 2011
                Categories
                Research Paper

                Life sciences
                phylogenetic diversity,chroogomphus rutilus,16s rrna,isolation,its
                Life sciences
                phylogenetic diversity, chroogomphus rutilus, 16s rrna, isolation, its

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